Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00064175-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00064175-M01, RRID:AB_2265746
- Product name
- Mouse Monoclonal LEPRE1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. LEPRE1 - leucine proline-enriched proteoglycan (leprecan) 1
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Expression characterization and functional implication of the collagen-modifying Leprecan proteins in mouse gonadal tissue and mature sperm.
Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues.
Proteomic dissection of the von Hippel-Lindau (VHL) interactome.
Severe osteogenesis imperfecta in cyclophilin B-deficient mice.
Prolyl 3-hydroxylase 1 deficiency causes a recessive metabolic bone disorder resembling lethal/severe osteogenesis imperfecta.
Zimmerman SM, Besio R, Heard-Lipsmeyer ME, Dimori M, Castagnola P, Swain FL, Gaddy D, Diekman AB, Morello R
AIMS genetics 2018;5(1):24-40
AIMS genetics 2018;5(1):24-40
Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues.
Ohe R, Aung NY, Meng H, Kabasawa T, Suto A, Tamazawa N, Yang S, Kato T, Yamakawa M
Leukemia & lymphoma 2016 Jul;57(7):1687-96
Leukemia & lymphoma 2016 Jul;57(7):1687-96
Proteomic dissection of the von Hippel-Lindau (VHL) interactome.
Lai Y, Song M, Hakala K, Weintraub ST, Shiio Y
Journal of proteome research 2011 Nov 4;10(11):5175-82
Journal of proteome research 2011 Nov 4;10(11):5175-82
Severe osteogenesis imperfecta in cyclophilin B-deficient mice.
Choi JW, Sutor SL, Lindquist L, Evans GL, Madden BJ, Bergen HR 3rd, Hefferan TE, Yaszemski MJ, Bram RJ
PLoS genetics 2009 Dec;5(12):e1000750
PLoS genetics 2009 Dec;5(12):e1000750
Prolyl 3-hydroxylase 1 deficiency causes a recessive metabolic bone disorder resembling lethal/severe osteogenesis imperfecta.
Cabral WA, Chang W, Barnes AM, Weis M, Scott MA, Leikin S, Makareeva E, Kuznetsova NV, Rosenbaum KN, Tifft CJ, Bulas DI, Kozma C, Smith PA, Eyre DR, Marini JC
Nature genetics 2007 Mar;39(3):359-65
Nature genetics 2007 Mar;39(3):359-65
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LEPRE1 Antibody (3C7) [H00064175-M01] - Analysis of LEPRE1 expression in transfected 293T cell line by LEPRE1 monoclonal antibody (M01), clone 3C7.Lane 1: LEPRE1 transfected lysate(46 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LEPRE1 Antibody (3C7) [H00064175-M01] - LEPRE1 monoclonal antibody (M01), clone 3C7 Analysis of LEPRE1 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: LEPRE1 Antibody (3C7) [H00064175-M01] - Detection limit for recombinant GST tagged LEPRE1 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: LEPRE1 Antibody (3C7) [H00064175-M01] - Analysis of monoclonal antibody to LEPRE1 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LEPRE1 Antibody (3C7) [H00064175-M01] - Analysis of monoclonal antibody to LEPRE1 on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 6 ug/ml.