- PA5-30157 - Invitrogen Antibodies RPL24 antibody

Submitted references Impaired dynamic interaction of axonal endoplasmic reticulum and ribosomes contributes to defective stimulus-response in spinal muscular atrophy.

Deng C, Reinhard S, Hennlein L, Eilts J, Sachs S, Doose S, Jablonka S, Sauer M, Moradi M, Sendtner M
Translational neurodegeneration 2022 Jun 2;11(1):31

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RPL24 using 30 µg of A549 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a RPL24 polyclonal antibody (Product # PA5-30157) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2) and NIH/3T3 (Lane 3). The blot was probed with Anti-RPL24 Polyclonal Antibody (Product # PA5-30157, 1 :1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 23 kDa band corresponding to RPL24 was observed across the cell lines tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of RPL24 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a RPL24 Polyclonal Antibody (Product # PA5-30157) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using RPL24 Polyclonal Antibody (Product # PA5-30157). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with RPL24 Polyclonal Antibody (Product # PA5-30157) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of RPL24 was achieved by transfecting HeLa cells with RPL24 specific siRNAs (Silencer® select Product # s12199, s12198). Western blot analysis (Fig. a) was performed using whole cell extracts from the RPL24 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with RPL24 Polyclonal Antibody (Product # PA5-30157, 1:2000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RPL24.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of RPL24 in paraformaldehyde-fixed HeLa cells using a RPL24 polyclonal antibody (Product # PA5-30157) at a 1:500 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: RPL24 Polyclonal Antibody detects RPL24 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RPL24 stained by RPL24 Polyclonal Antibody (Product # PA5-30157) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Ribosome responsiveness to the BDNF/TrkB signaling as well as ribosome/ER tethering are impaired in Smn-deficient axon terminals. a BDNF-stimulated Smn + / + ;SMN2tgtg and Smn -/- ;SMN2tgtg motoneurons expressing mCherry-KDEL were stained against RPL24, RPS6 and mCherry-KDEL. Growth cones were imaged by SIM. White boxes indicate enlarged ROIs within growth cones. In control but not Smn -/- ;SMN2tgtg motoneurons, BDNF induces formation of RPL24/RPS6 co-clusters that colocalize with the axonal ER at 10 s and 1-min poststimulation. b Smn -/- ;SMN2tgtg motoneurons do not exhibit increased number of RPL24/RPS6 co-clusters upon stimulation (n.s., P = 0.9999; n = 21-23 cells), in contrast to Smn + / + ;SMN2tgtg (* P = 0.0147 for no BDNF vs. 10-s BDNF; * P = 0.0210 for no BDNF vs. 1-min BDNF; n = 13-16 cells from 3 independent experiments). In control experiments, either RPL24 or RPS6 antibodies were omitted to exclude the crosstalk between channels. Colocalization analysis detected almost no co-clusters in the growth cones of control neurons (see also Additional file 1 : Fig. S3). c In Smn + / + ;SMN2tgtg motoneurons, the number of RPL24/RPS6 co-clusters that colocalize with ER increases significantly within 10 s and 1-min of stimulation (** P = 0.0089 for no BDNF vs. 10 s BDNF; ** P = 0.0092 for no BDNF vs. 1-min BDNF; n = 13-16 cells from 3 independent experiments). In Smn -/- ;SMN2tgtg motoneurons the number of RPL24/RPS6 co-clusters that associate with ER does not increase upon

PA5-30157

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