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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NB110-59722 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-59722, RRID:AB_2130796
- Product name
- Mouse Monoclonal CIP2A Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. p90 autoantigen (2G10)
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Antigen sequence
A 300 amino acid portion of the C-t
erminus.- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.8 mg/ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Long-lived mice with reduced growth hormone signaling have a constitutive upregulation of hepatic chaperone-mediated autophagy.
Constitutive CHK1 Expression Drives a pSTAT3-CIP2A Circuit that Promotes Glioblastoma Cell Survival and Growth.
Cancerous inhibitor of protein phosphatase 2A regulates cisplatin resistance in ovarian cancer.
ER stress-related ATF6 upregulates CIP2A and contributes to poor prognosis of colon cancer.
Feedback between E2F1 and CIP2A regulated by human papillomavirus E7 in cervical cancer: implications for prognosis.
MicroRNA-383-5p acts as a prognostic marker and inhibitor of cell proliferation in lung adenocarcinoma by cancerous inhibitor of protein phosphatase 2A.
Afatinib induces apoptosis in NSCLC without EGFR mutation through Elk-1-mediated suppression of CIP2A.
CIP2A is a candidate therapeutic target in clinically challenging prostate cancer cell populations.
CIP2A is overexpressed in osteosarcoma and regulates cell proliferation and invasion.
miR-375 activates p21 and suppresses telomerase activity by coordinately regulating HPV E6/E7, E6AP, CIP2A, and 14-3-3ζ.
CIP2A facilitates apoptotic resistance of fibroblast-like synoviocytes in rheumatoid arthritis independent of c-Myc expression.
Tumor suppressor miR-375 regulates MYC expression via repression of CIP2A coding sequence through multiple miRNA-mRNA interactions.
CIP2A expression is associated with synovial hyperplasia and invasive function of fibroblast-like synoviocytes in rheumatoid arthritis.
Overexpression and small molecule-triggered downregulation of CIP2A in lung cancer.
CIP2A is over-expressed in acute myeloid leukaemia and associated with HL60 cells proliferation and differentiation.
CIP2A is overexpressed in gastric cancer and its depletion leads to impaired clonogenicity, senescence, or differentiation of tumor cells.
Endicott SJ, Boynton DN Jr, Beckmann LJ, Miller RA
Autophagy 2021 Mar;17(3):612-625
Autophagy 2021 Mar;17(3):612-625
Constitutive CHK1 Expression Drives a pSTAT3-CIP2A Circuit that Promotes Glioblastoma Cell Survival and Growth.
Khanna A, Thoms JAI, Stringer BW, Chung SA, Ensbey KS, Jue TR, Jahan Z, Subramanian S, Anande G, Shen H, Unnikrishnan A, McDonald KL, Day BW, Pimanda JE
Molecular cancer research : MCR 2020 May;18(5):709-722
Molecular cancer research : MCR 2020 May;18(5):709-722
Cancerous inhibitor of protein phosphatase 2A regulates cisplatin resistance in ovarian cancer.
Li W, Zhang H, Yang L, Wang Y
Oncology letters 2019 Jan;17(1):1211-1216
Oncology letters 2019 Jan;17(1):1211-1216
ER stress-related ATF6 upregulates CIP2A and contributes to poor prognosis of colon cancer.
Liu CY, Hsu CC, Huang TT, Lee CH, Chen JL, Yang SH, Jiang JK, Chen WS, Lee KD, Teng HW
Molecular oncology 2018 Oct;12(10):1706-1717
Molecular oncology 2018 Oct;12(10):1706-1717
Feedback between E2F1 and CIP2A regulated by human papillomavirus E7 in cervical cancer: implications for prognosis.
Wang X, Gao P, Wang M, Liu J, Lin J, Zhang S, Zhao Y, Zhang J, Pan W, Sun Z, Sun F, Zhao W, Guo C, Wang Q
American journal of translational research 2017;9(5):2327-2339
American journal of translational research 2017;9(5):2327-2339
MicroRNA-383-5p acts as a prognostic marker and inhibitor of cell proliferation in lung adenocarcinoma by cancerous inhibitor of protein phosphatase 2A.
Zhao S, Gao X, Zang S, Li Y, Feng X, Yuan X
Oncology letters 2017 Sep;14(3):3573-3579
Oncology letters 2017 Sep;14(3):3573-3579
Afatinib induces apoptosis in NSCLC without EGFR mutation through Elk-1-mediated suppression of CIP2A.
Chao TT, Wang CY, Chen YL, Lai CC, Chang FY, Tsai YT, Chao CH, Shiau CW, Huang YC, Yu CJ, Chen KF
Oncotarget 2015 Feb 10;6(4):2164-79
Oncotarget 2015 Feb 10;6(4):2164-79
CIP2A is a candidate therapeutic target in clinically challenging prostate cancer cell populations.
Khanna A, Rane JK, Kivinummi KK, Urbanucci A, Helenius MA, Tolonen TT, Saramäki OR, Latonen L, Manni V, Pimanda JE, Maitland NJ, Westermarck J, Visakorpi T
Oncotarget 2015 Aug 14;6(23):19661-70
Oncotarget 2015 Aug 14;6(23):19661-70
CIP2A is overexpressed in osteosarcoma and regulates cell proliferation and invasion.
Zhai M, Cong L, Han Y, Tu G
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2014 Feb;35(2):1123-8
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2014 Feb;35(2):1123-8
miR-375 activates p21 and suppresses telomerase activity by coordinately regulating HPV E6/E7, E6AP, CIP2A, and 14-3-3ζ.
Jung HM, Phillips BL, Chan EK
Molecular cancer 2014 Apr 8;13:80
Molecular cancer 2014 Apr 8;13:80
CIP2A facilitates apoptotic resistance of fibroblast-like synoviocytes in rheumatoid arthritis independent of c-Myc expression.
Lee J, Jeong H, Park EJ, Hwang JW, Huang B, Bae EK, Ahn JK, Cha HS, Koh EM
Rheumatology international 2013 Sep;33(9):2241-8
Rheumatology international 2013 Sep;33(9):2241-8
Tumor suppressor miR-375 regulates MYC expression via repression of CIP2A coding sequence through multiple miRNA-mRNA interactions.
Jung HM, Patel RS, Phillips BL, Wang H, Cohen DM, Reinhold WC, Chang LJ, Yang LJ, Chan EK
Molecular biology of the cell 2013 Jun;24(11):1638-48, S1-7
Molecular biology of the cell 2013 Jun;24(11):1638-48, S1-7
CIP2A expression is associated with synovial hyperplasia and invasive function of fibroblast-like synoviocytes in rheumatoid arthritis.
Lee J, Park EJ, Hwang JW, Oh JM, Kim H, Bae EK, Choi YL, Han J, Ahn JK, Cha HS, Koh EM
Rheumatology international 2012 Jul;32(7):2023-30
Rheumatology international 2012 Jul;32(7):2023-30
Overexpression and small molecule-triggered downregulation of CIP2A in lung cancer.
Ma L, Wen ZS, Liu Z, Hu Z, Ma J, Chen XQ, Liu YQ, Pu JX, Xiao WL, Sun HD, Zhou GB
PloS one 2011;6(5):e20159
PloS one 2011;6(5):e20159
CIP2A is over-expressed in acute myeloid leukaemia and associated with HL60 cells proliferation and differentiation.
Wang J, Li W, Li L, Yu X, Jia J, Chen C
International journal of laboratory hematology 2011 Jun;33(3):290-8
International journal of laboratory hematology 2011 Jun;33(3):290-8
CIP2A is overexpressed in gastric cancer and its depletion leads to impaired clonogenicity, senescence, or differentiation of tumor cells.
Li W, Ge Z, Liu C, Liu Z, Björkholm M, Jia J, Xu D
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Jun 15;14(12):3722-8
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Jun 15;14(12):3722-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CIP2A Antibody (2G10) [NB110-59722] - Detection of CIP2A in HeLa whole cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CIP2A Antibody (2G10) [NB110-59722] - Analysis of CIP2A in NIH/3T3 cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CIP2A Antibody (2G10) [NB110-59722] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using CIP2A antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CIP2A Antibody (2G10) [NB110-59722] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using CIP2A antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
