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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00057510-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00057510-M01, RRID:AB_426037
- Product name
- XPO5 monoclonal antibody (M01), clone 2C5-1B3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant XPO5.
- Antigen sequence
MRLSQKWQVINQRSLLCGEDEAADENPESQEMLEE
QLVRMLTREVMDLITVCCVSKKGADHSSAPPADGD
DEEMMATEVTPSAMAELTDLGKCLMKHEDVCTALL
ITAFNSLAWKDTLSCQRTTSQLCWPLLKQVLSGTL
LADAVTWLFTSVLKGLQMHGQHDGCMASLVHLAFQ
IYEALRPRYLEIRAVMEQIPEIQKDSLDQFDCKLL
NPSLQKVADKRRKDQFKRLIAGCIGKPLGEQFRKE
VHIKNLPSLFKKTKPMLETEVLDNDGGGLATIFEP- Isotype
- IgG
- Antibody clone number
- 2C5-1B3
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Knock-down of core proteins regulating microRNA biogenesis has no effect on sensitivity of lung cancer cells to ionizing radiation.
MicroRNAs are expressed and processed by human primary macrophages.
Surova O, Akbar NS, Zhivotovsky B
PloS one 2012;7(3):e33134
PloS one 2012;7(3):e33134
MicroRNAs are expressed and processed by human primary macrophages.
Luers AJ, Loudig OD, Berman JW
Cellular immunology 2010;263(1):1-8
Cellular immunology 2010;263(1):1-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- XPO5 monoclonal antibody (M01), clone 2C5-1B3 Western Blot analysis of XPO5 expression in Hela S3 NE ( Cat # L013V3 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of XPO5 expression in transfected 293T cell line by XPO5 monoclonal antibody (M01), clone 2C5-1B3.Lane 1: XPO5 transfected lysate(136.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged XPO5 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to XPO5 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of XPO5 transfected lysate using anti-XPO5 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with XPO5 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
