PA5-89201

antibody from Invitrogen Antibodies

Targeting: SSNA1 N14, NA14

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA5-89201

Provider

Invitrogen Antibodies

Product name

SSNA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MTQQGAALQN YNNELVKCIE ELCQKREELC RQIQEEEDEK QRLQNEVRQL TEKLARVNEN LARKIASRNE FDRTIAETEA AYLKILESSQ TLLSVLKREA GNLTKATAPD QKSSGGRDS; Positive Samples: Mouse spleen; Cellular Location: Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.71 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of mouse spleen, using SSNA1 Polyclonal antibody (Product # PA5-89201) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SSNA1 in extracts of mouse spleen using SSNA1 Polyclonal Antibody (Product # PA5-89201) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SSNA1 was performed in A549 cells using SSNA1 Polyclonal Antibody (Product # PA5-89201).