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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-31214 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DPPA4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, human ESC.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DPPA4 Polyclonal Antibody (Product # PA5-31214). Sample (20 µg of whole cell lysate). Lane A: human ESC. 12% SDS PAGE. DPPA4 Polyclonal Antibody (Product # PA5-31214) diluted at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DPPA4 Polyclonal Antibody (Product # PA5-31214). Sample (30 µg of whole cell lysate). Lane A: NT2D1. 12% SDS PAGE. DPPA4 Polyclonal Antibody (Product # PA5-31214) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DPPA4 in paraformaldehyde-fixed Human ESC cells using a DPPA4 polyclonal antibody (Product # PA5-31214) at a 1:400 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of DPPA4 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: DPPA4 Polyclonal Antibody (Product # PA5-31214) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H661 xenograft, using DPPA4 (Product # PA5-31214) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
