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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PAB12505 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12505, RRID:AB_10551170
- Product name
- AANAT polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against AANAT.
- Storage
- Store at 4°C for short term. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Arylalkylamine N-acetyltransferase: "the Timezyme".
Melatonin synthesis enzymes in Macaca mulatta: focus on arylalkylamine N-acetyltransferase (EC 2.3.1.87).
Retinal melatonin production: role of proteasomal proteolysis in circadian and photic control of arylalkylamine N-acetyltransferase.
14-3-3 Proteins and photoneuroendocrine transduction: role in controlling the daily rhythm in melatonin.
cAmp regulation of arylalkylamine N-acetyltransferase (AANAT, EC 2.3.1.87): a new cell line (1E7) provides evidence of intracellular AANAT activation.
Klein DC
The Journal of biological chemistry 2007 Feb 16;282(7):4233-7
The Journal of biological chemistry 2007 Feb 16;282(7):4233-7
Melatonin synthesis enzymes in Macaca mulatta: focus on arylalkylamine N-acetyltransferase (EC 2.3.1.87).
Coon SL, Del Olmo E, Young WS 3rd, Klein DC
The Journal of clinical endocrinology and metabolism 2002 Oct;87(10):4699-706
The Journal of clinical endocrinology and metabolism 2002 Oct;87(10):4699-706
Retinal melatonin production: role of proteasomal proteolysis in circadian and photic control of arylalkylamine N-acetyltransferase.
Iuvone PM, Brown AD, Haque R, Weller J, Zawilska JB, Chaurasia SS, Ma M, Klein DC
Investigative ophthalmology & visual science 2002 Feb;43(2):564-72
Investigative ophthalmology & visual science 2002 Feb;43(2):564-72
14-3-3 Proteins and photoneuroendocrine transduction: role in controlling the daily rhythm in melatonin.
Klein DC, Ganguly S, Coon S, Weller JL, Obsil T, Hickman A, Dyda F
Biochemical Society transactions 2002 Aug;30(4):365-73
Biochemical Society transactions 2002 Aug;30(4):365-73
cAmp regulation of arylalkylamine N-acetyltransferase (AANAT, EC 2.3.1.87): a new cell line (1E7) provides evidence of intracellular AANAT activation.
Coon SL, Weller JL, Korf HW, Namboodiri MA, Rollag M, Klein DC
The Journal of biological chemistry 2001 Jun 29;276(26):24097-107
The Journal of biological chemistry 2001 Jun 29;276(26):24097-107
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of AANAT using AANAT polyclonal antibody (Cat # PAB12505). Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- AANAT in COS-7 and 1E7 cells detected by ICC using AANAT polyclonal antibody (PAB12505). Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS-7 cells treated with FSK for 3 h.
- Validation comment
- Immunocytochemistry
