- 14-9771-37 - Invitrogen Antibodies COL10A1 antibody

Submitted references Spatiotemporal distribution of thrombospondin-4 and -5 in cartilage during endochondral bone formation and repair.

Andrés Sastre E, Maly K, Zhu M, Witte-Bouma J, Trompet D, Böhm AM, Brachvogel B, van Nieuwenhoven CA, Maes C, van Osch GJVM, Zaucke F, Farrell E
Bone 2021 Sep;150:115999

The Effects of Fluvastatin on Indian Hedgehog Pathway in Endochondral Ossification.

Ishikawa M, Ishii T, Morikawa T, Iijima Y, Sueishi K
Cartilage 2021 Dec;13(2_suppl):304S-314S

Angiogenic Potential of Tissue Engineered Cartilage From Human Mesenchymal Stem Cells Is Modulated by Indian Hedgehog and Serpin E1.

Nossin Y, Farrell E, Koevoet WJLM, Somoza RA, Caplan AI, Brachvogel B, van Osch GJVM
Frontiers in bioengineering and biotechnology 2020;8:327

Control of skeletal morphogenesis by the Hippo-YAP/TAZ pathway.

Vanyai HK, Prin F, Guillermin O, Marzook B, Boeing S, Howson A, Saunders RE, Snoeks T, Howell M, Mohun TJ, Thompson B
Development (Cambridge, England) 2020 Nov 12;147(21)

Perlecan Knockdown Significantly Alters Extracellular Matrix Composition and Organization During Cartilage Development.

Ocken AR, Ku MM, Kinzer-Ursem TL, Calve S
Molecular & cellular proteomics : MCP 2020 Jul;19(7):1220-1235

Impact of the Sensory and Sympathetic Nervous System on Fracture Healing in Ovariectomized Mice.

Niedermair T, Straub RH, Brochhausen C, Grässel S
International journal of molecular sciences 2020 Jan 8;21(2)

Lipid phosphatase SHIP-1 regulates chondrocyte hypertrophy and skeletal development.

So EY, Sun C, Wu KQ, Driesman A, Leggett S, Isaac M, Spangler T, Dubielecka-Szczerba PM, Reginato AM, Liang OD
Journal of cellular physiology 2020 Feb;235(2):1425-1437

Promoting Effect of Basic Fibroblast Growth Factor in Synovial Mesenchymal Stem Cell-Based Cartilage Regeneration.

Okamura G, Ebina K, Hirao M, Chijimatsu R, Yonetani Y, Etani Y, Miyama A, Takami K, Goshima A, Yoshikawa H, Ishimoto T, Nakano T, Hamada M, Kanamoto T, Nakata K
International journal of molecular sciences 2020 Dec 30;22(1)

Phenotypic Characterization of Bone Marrow Mononuclear Cells and Derived Stromal Cell Populations from Human Iliac Crest, Vertebral Body and Femoral Head.

Herrmann M, Hildebrand M, Menzel U, Fahy N, Alini M, Lang S, Benneker L, Verrier S, Stoddart MJ, Bara JJ
International journal of molecular sciences 2019 Jul 14;20(14)

Oral administration of EP4-selective agonist KAG-308 suppresses mouse knee osteoarthritis development through reduction of chondrocyte hypertrophy and TNF secretion.

Murahashi Y, Yano F, Chijimatsu R, Nakamoto H, Maenohara Y, Amakawa M, Miyake Y, Yamanaka H, Iba K, Yamashita T, Tanaka S, Saito T
Scientific reports 2019 Dec 30;9(1):20329

Identification of stromal ColXα1 and tumor-infiltrating lymphocytes as putative predictive markers of neoadjuvant therapy in estrogen receptor-positive/HER2-positive breast cancer.

Brodsky AS, Xiong J, Yang D, Schorl C, Fenton MA, Graves TA, Sikov WM, Resnick MB, Wang Y
BMC cancer 2016 Apr 18;16:274

A Hydrogel Model Incorporating 3D-Plotted Hydroxyapatite for Osteochondral Tissue Engineering.

Bartnikowski M, Akkineni AR, Gelinsky M, Woodruff MA, Klein TJ
Materials (Basel, Switzerland) 2016 Apr 14;9(4)

The gonadotrophin-releasing hormone receptor of alpha T3-1 pituitary cells regulates cellular levels of both of the phosphoinositidase C-linked G proteins, Gq alpha and G11 alpha, equally.

Shah BH, Milligan G
Molecular pharmacology 1994 Jul;46(1):1-7

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry of formalin-fixed paraffin embedded human infiltrating ductal carcinoma tissue using 10 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 10 µg/mL of Anti-Collagen Type X Purified (right) followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.

Supportive validation

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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 7 In vivo osteochondral repair in a mouse model: evaluation of chondrogenic repair ( a ) Histology of the tissue stained with toluidine blue (bar = 500 um). ( b ) Histology of the tissue stained with Saf-O (left columns; bar = 500 um) and the corresponding high-magnification images (right columns; bar = 100 um). ( c ) Immunohistochemical analysis of repair cartilage stained for COL2 (bar = 500 um). ( d ) Immunohistochemical analysis of repair tissue stained for COL10 (bar = 500 um). bFGF, basic fibroblast growth factor; Saf-O, Safranin-O; COL2, collagen type II; COL10, collagen type X.

Supportive validation

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Experimental details: Figure 5 Hypertrophic cartilage area and number of TRAP-positive callus cells . ( A , B ) Number/area of hypertrophic chondrocytes, visualized by collagen X staining, in Tac1-/-, alpha-CGRP-/- ( A ), SYX ( B ), and WT mice ( A , B ) at the time points 5, 9, 13, 16, and 21 days after fracture. Values were calculated as a percentage of total callus area and are presented as mean +- SD. * in black indicates differences between neurotransmitter deficient mice and WT animals. ( C , D ) Number of TRAP-positive cells in the callus tissue of Tac1-/-, alpha-CGRP-/- ( C ), SYX ( D ), and WT mice ( C , D ) at the time points 5, 9, 13, 16, and 21 days after fracture. The number of cells was calculated in relation to the total area of callus tissue as number/mm 2 . ( E ) Representative image of collagen X stained hypertrophic callus area of an alpha-CGRP-/- mouse 13 days after fracture. ( F ) Representative image of TRAP-positive callus cells 16 days after fracture in the callus tissue of a WT mouse. Overview images scanned with 20x magnification (TissueFAXSi plus). Red boxes demonstrate the enlarged view. Red arrow points to stained cells. Col X = collagen X; B = bone, C = Callus tissue, F = Fracture site, M = Muscle. * = p

Supportive validation

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Experimental details: FIGURE 1 Chondrogenic differentiation of bone marrow derived mesenchymal stem cells (BMSC). (A) Histological and Immunohistological staining of day 21 chondrogenically differentiated BMSC-pellets of the donors for Collagen type II, Thionine and Collagen type X. (B) qPCR Gene-expression analysis of chondrocyte and hypertrophy marker genes in day 21 chondrogenically differentiated BMSCs. Raw average Ct value for COL2A1 = 20.54; COL10A1 = 20.99; ALPL = 28.19.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 5 Effects of KAG-308 on chondrocyte hypertrophy in vitro . ( a ) mRNA levels of Col10a1 , Runx2 , Mef2c , and Col2a1 in pellet culture of mouse articular chondrocytes treated with indicated concentration of KAG-308 for 2 wks. Symbols represent individual pellets; long and short bars show the mean and SD, respectively. * P < 0.05, ** P < 0.005, *** P < 0.0005 vs vehicle by ANOVA followed by Dunnett's post hoc test. ( b ) Safranin O staining and Col10a1 immunostaining of cultured pellets of mouse articular chondrocytes treated with KAG-308 for 2 wks. Inset boxes in safranin-O staining indicate enlarged images. Scale bar, 200 um. ( c ) Immunoblotting of Hdac4 in nuclear and cytosolic fractions from mouse articular chondrocytes in a time course with 10 nM KAG-308 treatment. Right graphs indicate quantitative densitometry analysis of the immunoblots (n = 5). Hdac4 values were normalized to Pcna (nuclear) or Actin (cytosol). Long and short bars show the mean and SD, respectively. * P < 0.05, ** P < 0.005 vs 0 hour by ANOVA followed by Dunnett's post hoc test. ( d ) Immunocytochemistry of Hdac4 in mouse articular chondrocytes after 4-d-culture in BMP-2-containing medium with vehicle or 10 nM KAG-308. Right graph shows the percent of cells with nuclear-localized Hdac4. Symbols represent independent slides; long and short bars show the mean and SD, respectively. * P < 0.0005 by Welch's t test. Scale bar, 20 um.

Supportive validation

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Experimental details: Figure 5 Accelerated chondrogenesis in SHIP-1 KO PalphaS MSCs. PalphaS MSC pellets differentiated in vitro for 21 days using the Mouse StemXVivo Chondrogenic Base Media and Supplement were fixed, paraffin embedded, sectioned and IHC-stained. (a and b) Sections from WT (a) and SHIP-1 KO (b) PalphaS MSC pellets were stained with an antibody against the early chondrogenic lineage marker Col II. Bound antibody was visualized in bright red with a NorthernLights 557-conjugated anti-sheep secondary antibody. (c and d) Sections from WT (c) and SHIP-1 KO (d) PalphaS MSC pellets were stained with a bright red eFluor570-conjugated antibody against the hypertrophic chondrocyte lineage marker Col X. (e and f) Sequential sections from the same WT cell pellet as in (c) and from the same SHIP-1 KO cell pellet as in (d) were subjected to H&E staining. Magnification: x200 (a-d); x400 (e and f). Scale bar = 50 mum. (g) Left panel, the difference in cell sizes between WT and SHIP-1 KO PalphaS MSCs after chondrogenic induction was quantified by using the NIH ImageJ program and indicated as ImageJ Area Units. Middle and right panels, gene expression levels of Col2a1 and Col10a1 were evaluated by quantitative real-time PCR. GAPDH expression was assessed as an internal reference for quantification. Gene expression levels were expressed as relative fold increase over the internal control. Student's t test was performed and p < .05 is considered significant. Col II, collagen type II; Col X, collagen t

Supportive validation

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Experimental details: Figure 8 Immunofluorescence images illustrating deep and surface sections. Staining of collagen I (red), collagen II (green), aggrecan (green) and collagen X (green) was performed on constructs from all groups on day 28 (as indicated; DAPI nuclei staining in light blue); ( A , E , I ): GelMA-ALG; ( B , F , J ): GelMA/HAMA-ALG; ( C , G , K ): GelMA-ALG/HAP and ( D , H , L ): GelMA/HAMA-ALG/HAP. Images were taken from the deep hydrogel zone near to the ALG or ALG/HAP scaffold, with surface images inset; scale bar 50 um.

14-9771-37

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