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LearnAF5450
antibody from Novus Biologicals
Targeting: ATG3
APG3L, DKFZp564M1178, FLJ22125, MGC15201, PC3-96
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AF5450 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal ATG3/APG3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human, mouse and rat ATG3 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Escitalopram oxalate induces apoptosis in U-87MG cells and autophagy in GBM8401 cells.
Chen VC, Hsieh YH, Chen LJ, Hsu TC, Tzang BS
Journal of cellular and molecular medicine 2018 Feb;22(2):1167-1178
Journal of cellular and molecular medicine 2018 Feb;22(2):1167-1178
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat ATG3 by Western Blot. Western blot shows lysates of human brain tissue, mouse brain tissue, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat ATG3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5450) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ATG3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
