AF7560

antibody from R&D Systems

Targeting: GLRX3 bA500G10.4, GLRX4, GRX3, GRX4, PICOT, TXNL2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF7560

Provider

R&D Systems

Product name

Human/Mouse/Rat Glutaredoxin 3/GLRX3 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human, mouse and rat Glutaredoxin 3/GLRX3 in Western blots.

Reactivity

Human, Mouse, Rat

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

O76003


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human, Mouse, and Rat Glutaredoxin 3/GLRX3 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Glutaredoxin 3/GLRX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7560) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glutaredoxin 3/GLRX3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Glutaredoxin 3/GLRX3 in Human Breast Cancer Tissue. Glutaredoxin 3/GLRX3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human/Mouse/Rat Glutaredoxin 3/GLRX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7560) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in ductal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.