Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB7560 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Glutaredoxin 3/GLRX3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Glutaredoxin 3/GLRX3 in ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O76003- Isotype
- IgG
- Antibody clone number
- 791431
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Glutaredoxin 3/ GLRX3 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Glutaredoxin 3/GLRX3 Monoclonal Antibody (Catalog # MAB7560) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutaredoxin 3/GLRX3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Glutaredoxin 3/GLRX3 in NIH-3T3 Mouse Cell Line. Glutaredoxin 3/GLRX3 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human Glutaredoxin 3/ GLRX3 Monoclonal Antibody (Catalog # MAB7560) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
