TA805678

antibody from OriGene

Targeting: ROS1 c-ros-1, MCF3, ROS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA805678

Provider

OriGene

Proper citation

OriGene Cat#TA805678, RRID:AB_2627825

Product name

ROS1 mouse monoclonal antibody, clone OTI1F3 (formerly 1F3)

Antibody type

Monoclonal

Description

ROS1 mouse monoclonal antibody, clone OTI1F3 (formerly 1F3)

Host

Mouse

Conjugate

Unconjugated

Epitope

ROS1

Isotype

IgG

Antibody clone number

OTI1F3

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROS1 (RC220652, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ROS1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ROS1 monoclonal antibody.(1:500)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunocytochemistry staining of HCC78 cells using anti-ROS1 mouse monoclonal antibody (TA805678)(Left). The right is HELA cells as negative control.(1:2000)

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of ROS1 positive Human lung tissue using anti-ROS1 mouse monoclonal antibody. (TA805678; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of ROS1 positive Human lung tissue using anti-ROS1 mouse monoclonal antibody. (TA805678; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of ROS1 positive Human lung tissue using anti-ROS1 mouse monoclonal antibody. (TA805678; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded HCC78 (left) and HeLa (right) xenograft using TA805678 (0.2ug/ml). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min)

Validation comment

IHC