MA5-29334

antibody from Invitrogen Antibodies

Targeting: HYOU1 Grp170, HSP12A, ORP150

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29334

Provider

Invitrogen Antibodies

Product name

HYOU1 Recombinant Rabbit Monoclonal Antibody (201)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Antibody clone number

201

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HYOU1 in Lane A: 293T Whole Cell Lysate (30 µg), Lane B: Hela Whole Cell Lysate (30 µg) . Samples were probed using a HYOU1 Monoclonal Antibody (Product # MA5-29334) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:111 kDa. Observed band size:170 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using HYOU1 Recombinant Rabbit Monoclonal Antibody (201) (Product # MA5-29334) at 1:500 dilution. Lane A: 293T Whole Cell Lysate, Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 111 kDa. Observed band size: 170 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of ORP150 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with HYOU1 Recombinant Rabbit Monoclonal Antibody (201) (Product # MA5-29334, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human HYOU1 in human colon carcinoma with HYOU1 Recombinant Rabbit Monoclonal Antibody (201) (Product # MA5-29334, 1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to colonic gland.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HYOU1 Immunoprecipitation using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate, Lane B: 0.5 mg 293T Whole Cell Lysate, Lane C: 0.5 mg Hela Whole Cell Lysate 2 µL with HYOU1 Recombinant Rabbit Monoclonal Antibody (201) (Product # MA5-29334) and 15 µL of 50 % Protein G agarose. Primary antibody: HYOU1 Recombinant Rabbit Monoclonal Antibody (201), at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 111 kDa. Observed band size: 111 kDa.