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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- 20777-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HPDL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAAPALRLC HIAFHVPAGQ PLARNLQRLF GFQPLASREV DGWRQLALRS GDAVFLVNEG AGSGEPLYGL DPRHAVPSAT NLCFDVADAG AATRELAALG CSVPVPPVRV RDAQGAATYA VVSSPAGILS LTLLERAGYR GPFLPGFRPV SSAPGPGWVS RVDHLTLACT PGSSPTLLRW FHDCLGFCHL PLSPGEDPEL GLEMTAGFGL GGLRLTALQA QPGSIVPTLV LAESLPGATT RQDQVEQFLA RHKGPGLQHV GLYTPNIVEA TEGVATAGGQ FLAPPGAYYQ QPGKERQIRA AGHEPHLLAR QGILLDGDKG KFLLQVFTKS LFTEDTFFLE LIQRQGATGF GQGNIRALWQ SVQEQSARSQ EA (1-371 aa encoded by BC007293)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 20777-1-AP (HPDL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 20777-1-AP (HPDL antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using HPDL antibody 20777-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 40x lens).
