Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ABIN954399 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Queuine tRNA-Ribosyltransferase Domain Containing 1 (QTRTD1) (C-Term), (AA 328-359) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 328-359 amino acids from the C-terminal region of Human QTRTD1. Genename: QTRTD1
- Description
- Affinity Chromatography on Protein A
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term,AA 328-359
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Characterization of the human tRNA-guanine transglycosylase: confirmation of the heterodimeric subunit structure.
hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes.
Chen YC, Kelly VP, Stachura SV, Garcia GA
RNA (New York, N.Y.) 2010 May;16(5):958-68
RNA (New York, N.Y.) 2010 May;16(5):958-68
hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes.
Lamesch P, Li N, Milstein S, Fan C, Hao T, Szabo G, Hu Z, Venkatesan K, Bethel G, Martin P, Rogers J, Lawlor S, McLaren S, Dricot A, Borick H, Cusick ME, Vandenhaute J, Dunham I, Hill DE, Vidal M
Genomics 2007 Mar;89(3):307-15
Genomics 2007 Mar;89(3):307-15
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of QTRTD1 Antibody (C-term) Cat.-No AP53533PU-N in MCF-7 cell line lysates (35ug/lane). This demonstrates the QTRTD1 antibody detected the QTRTD1 protein (arrow
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of QTRTD1 Antibody (C-term) Cat.-No AP53533PU-N with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Fixed and paraffin embedded human breast tissue stained QTRTD1 Antibody (C-term) Cat.-No AP53533PU-N with followed by peroxidase conjugation of the secondary antibody and DAB staining
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using QTRTD1 Antibody (C-term) Cat.-No AP53533PU-N (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.