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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB7065 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7065, RRID:AB_1575285
- Product name
- GPX4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of GPX4.
- Antigen sequence
C-EEPLVIEKDLPHY- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mammalian selenoprotein thioredoxin-glutathione reductase. Roles in disulfide bond formation and sperm maturation.
Su D, Novoselov SV, Sun QA, Moustafa ME, Zhou Y, Oko R, Hatfield DL, Gladyshev VN
The Journal of biological chemistry 2005 Jul 15;280(28):26491-8
The Journal of biological chemistry 2005 Jul 15;280(28):26491-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- GPX4 polyclonal antibody (Cat # PAB7065) (0.1 ug/mL) staining of human testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- GPX4 polyclonal antibody (Cat # PAB7065, 0.3 ug/mL) staining of paraffin embedded human testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
