Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00008702-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00008702-M01, RRID:AB_537058
- Product name
- Mouse Monoclonal B4GALT4 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. B4GALT4 - UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: B4GALT4 Antibody (5E2) [H00008702-M01] - Analysis of B4GALT4 over-expressed 293 cell line, cotransfected with B4GALT4 Validated Chimera RNAi ( Cat # H00008702-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with B4GALT4 monoclonal antibody (M01), clone 5E2 (Cat # H00008702-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: B4GALT4 Antibody (5E2) [H00008702-M01] - Analysis of B4GALT4 expression in transfected 293T cell line by B4GALT4 monoclonal antibody (M01), clone 5E2.Lane 1: B4GALT4 transfected lysate(40 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: B4GALT4 Antibody (5E2) [H00008702-M01] - Detection limit for recombinant GST tagged B4GALT4 is approximately 0.1ng/ml as a capture antibody.
