Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 675202 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#675202, RRID:AB_2565493
- Product name
- Purified anti-NLRP12
- Antibody type
- Monoclonal
- Antigen
- Partial human NLRP12 recombinant protein (93-1061 a.a.) expressed in E. coli.
- Description
- NLRP12, a member of the Nod-like receptor (NLR) family of proteins, was originally suggested to act as an inflammasome forming component and a modulator of NF-κB signaling. Recent studies revealed that NLRP12 plays anti-inflammatory roles, as NLRP12-deficient mice are more susceptible to chemically induced colitis, and show a more severe infiltration of granulocytes and T cells. The colitis induced in NLRP12-/- mice is associated with increased production of proinflammatory cytokines and activation of both NF-κB and ERK signaling pathways. NLRP12 was reported to attenuate canonical NF-κB signaling by inhibiting IRAK-1 phosphorylation. NLRP12 also down-regulates non-canonical NF-κB signaling through interaction with TRAF3 and the NF-κB inducing kinase NIK.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 3D11A53
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate from HL-60 cells (lane 1) or DMSO treated HL-60 cells (1.25% DMSO treated for five days, lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-NLRP12 (clone 3D11A53) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated