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antibody from Invitrogen Antibodies
Targeting: PRDX5
ACR1, AOEB166, B166, MGC117264, MGC142283, MGC142285, PLP, PMP20, PRDX6, PRXV, SBBI10
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- PA5-79864 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRDX5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRDX5 in Lane 1: A549 whole cell lysate, Lane 2: rat brain tissue lysate, Lane 3: mouse brain tissue lysate using 40-50 µg per well. Sample was incubated with PRDX5 (Product # PA5-79864) at a dilution of 0.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of PRDX5 in SMMC cells. Sample was incubated with PRDX5 polyclonal antibody (Product # PA5-79864).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Peroxiredoxin 5 using anti-Peroxiredoxin 5 antibody (Product # PA5-79864) . Peroxiredoxin 5 was detected in a section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Peroxiredoxin 5 antibody (Product # PA5-79864) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PRDX5 on paraffin-embedded rat intestine tissue. Sample was incubated with PRDX5 polyclonal antibody (Product# PA5-79864).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PRDX5 on paraffin-embedded human lung cancer tissue. Sample was incubated with PRDX5 polyclonal antibody (Product# PA5-79864).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PRDX5 on paraffin-embedded mouse intestine tissue. Sample was incubated with PRDX5 polyclonal antibody (Product# PA5-79864).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry of PRDX5 in A549 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with PRDX5 Polyclonal Antibody (Product # PA5-79864) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.
