PA5-93329

antibody from Invitrogen Antibodies

Targeting: SOD3 EC-SOD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-93329

Provider

Invitrogen Antibodies

Product name

SOD3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: MLALLCSCLL LAAGASDAWT GEDSAEPNSD SAEWIRDMYA KVTEIWQEVM QRRDDDGALH AACQVQPSAT LDAAQPRVTG VVLFRQLAPR AKLDAFFALE

Concentration

0.96 mg/mL

References

Submitted references

N-Acetylcysteine Enhances the Recovery of Ischemic Limb in Type-2 Diabetic Mice.

Zhu Q, Liu X, Zhu Q, Liu Z, Yang C, Wu H, Zhang L, Xia X, Wang M, Hao H, Cui Y, Zhang G, Hill MA, Flaker GC, Zhou S, Liu Z
Antioxidants (Basel, Switzerland) 2022 May 31;11(6)

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Combination of Antioxidant Enzyme Overexpression and N-Acetylcysteine Treatment Enhances the Survival of Bone Marrow Mesenchymal Stromal Cells in Ischemic Limb in Mice With Type 2 Diabetes.

Zhu Q, Hao H, Xu H, Fichman Y, Cui Y, Yang C, Wang M, Mittler R, Hill MA, Cowan PJ, Zhang G, He X, Zhou S, Liu Z
Journal of the American Heart Association 2021 Oct 5;10(19):e023491

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using SOD3 Polyclonal antibody (Product # PA5-93329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SOD3 in extracts of various cell lines using SOD3 Polyclonal Antibody (Product # PA5-93329) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Extracellular superoxide dismutase [Cu-Zn] was performed using 70% confluent log phase T-47D and Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SOD3 Polyclonal Antibody (Product # PA5-93329) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytoplasmic localization in T-47D cells but not in HepG2 cells (Panel e) which is reported to be low to negative for SOD3 expression. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Advanced glycation end products (AGEs) impaired the viability of bone marrow mesenchymal stromal cells (bMSCs) through increased reactive oxygen species (ROS) production attributable to selective reduction of superoxide dismutase (SOD)-1 and SOD-3 expression. A through C , Culture of bMSCs with AGE-BSA (with concentration from 100 to 400 mug/mL with BSA as control) significantly increased intracellular ROS ( A ) and early ( B ) and late apoptosis ( C ) in a dose-dependent manner. n=6/group. * P