H00003081-M11

antibody from Abnova Corporation

Targeting: HGD AKU, HGO

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003081-M11

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003081-M11, RRID:AB_1575941

Product name

HGD monoclonal antibody (M11), clone 1F1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant HGD.

Antigen sequence

MAELKYISGFGNECSSEDPRCPGSLPEGQNNPQVC
PYNLYAEQLSGSAFTCPRSTNKRSWLYRILPSVSH
KPFESIDEGHVTHNWDEVDPDPNQLRWKPFEIPKA
SQKKVDFVSGLHTLCGAGDIKSNNGLAIHIFLCNT
SMENRCFYNSDGDFLIVPQKGNLLIYTEFGKMLVQ
PNEICVIQRGMRFSIDVFEETRGYILEVYGVHLEL
PDLGPIGANGLANPRDFLIPIAWYEDRQVPGGYTV
INKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLK
NFMVINSVAFDHADPSIFTVLTALRRPARSSWHLR
GLPMAPWHLCLNHL


Isotype

IgG

Antibody clone number

1F1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HGD expression in transfected 293T cell line by HGD monoclonal antibody (M11), clone 1F1.Lane 1: HGD transfected lysate(50 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged HGD is 3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of HGD transfected lysate using anti-HGD monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HGD MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol