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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00003081-M11 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003081-M11, RRID:AB_2118600
- Product name
- Mouse Monoclonal HGD Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. HGD - homogentisate 1,2-dioxygenase (homogentisate oxidase) (1F1)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HGD Antibody (1F1) [H00003081-M11] - Analysis of HGD expression in transfected 293T cell line by HGD monoclonal antibody (M11), clone 1F1. Lane 1: HGD transfected lysatE (50 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: HGD Antibody (1F1) [H00003081-M11] - Detection limit for recombinant GST tagged HGD is 3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: HGD Antibody (1F1) [H00003081-M11] - Analysis of HGD transfected lysate using anti-HGD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HGD rabbit polyclonal antibody.
