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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB4852 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4852, RRID:AB_1572551
- Product name
- CCNE1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CCNE1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references G1 arrest by p16INK4A uncouples growth from cell cycle progression in leukemia cells with deregulated cyclin E and c-Myc expression.
The structure of cyclin E1/CDK2: implications for CDK2 activation and CDK2-independent roles.
The tumor-specific hyperactive forms of cyclin E are resistant to inhibition by p21 and p27.
Ausserlechner MJ, Obexer P, Geley S, Kofler R
Leukemia 2005 Jun;19(6):1051-7
Leukemia 2005 Jun;19(6):1051-7
The structure of cyclin E1/CDK2: implications for CDK2 activation and CDK2-independent roles.
Honda R, Lowe ED, Dubinina E, Skamnaki V, Cook A, Brown NR, Johnson LN
The EMBO journal 2005 Feb 9;24(3):452-63
The EMBO journal 2005 Feb 9;24(3):452-63
The tumor-specific hyperactive forms of cyclin E are resistant to inhibition by p21 and p27.
Wingate H, Zhang N, McGarhen MJ, Bedrosian I, Harper JW, Keyomarsi K
The Journal of biological chemistry 2005 Apr 15;280(15):15148-57
The Journal of biological chemistry 2005 Apr 15;280(15):15148-57
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CCNE1 polyclonal antibody (Cat # PAB4852) in A2058 cell lysate (35 ug/lane). CCNE1 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human testisreacted with CCNE1 polyclonal antibody (Cat # PAB4852), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H292 cells using CCNE1 polyclonal antibody (Cat # PAB4852)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
