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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- GTX104990 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104990, RRID:AB_1950954
- Product name
- MTHFD2 antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample (30 ?g of whole cell lysate) A: 293T B: A431 (GTX27909) C: H1299 D: HeLa E: HepG2 (GTX27900) F: Molt-4 (GTX27912) G: Raji 10% SDS PAGE GTX104990 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MTHFD2(GTX104990) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of MTHFD2 protein from 293T whole cell extracts using 5 £gg of MTHFD2 antibody [N1C3] (GTX104990).Western blot analysis was performed using MTHFD2 antibody [N1C3] (GTX104990).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SAS xenograft, using MTHFD2(GTX104990) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human MCF7 xenograft. MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:500.
