12270-1-AP

antibody from Invitrogen Antibodies

Targeting: MTHFD2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12270-1-AP

Provider

Invitrogen Antibodies

Product name

MTHFD2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAATSLMSA LAARLLQPAH SCSLRLRPFH LAAVRNEAVV ISGRKLAQQI KQEVRQEVEE WVASGNKRPH LSVILVGENP ASHSYVLNKT RAAAVVGINS ETIMKPASIS EEELLNLINK LNNDDNVDGL LVQLPLPEHI DERRICNAVS PDKDVDGFHV INVGRMCLDQ YSMLPATPWG VWEIIKRTGI PTLGKNVVVA GRSKNVGMPI AMLLHTDGAH ERPGGDATVT ISHRYTPKEQ LKKHTILADI VISAAGIPNL ITADMIKEGA AVIDVGINRV HDPVTAKPKL VGDVDFEGVR QKAGYITPVP GGVGPMTVAM LMKNTIIAAK KVLRLEEREV LKSKELGVAT N (1-350 aa encoded by BC017054)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 12270-1-AP (MTHFD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12270-1-AP (MTHFD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 12270-1-AP (MTHFD2 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using (Product # 12270-1-AP) (MTHFD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MTHFD2 (IP:12270-1-AP, 4ug; Detection:12270-1-AP 1:300) with mouse testis tissue lysate 4000ug.