Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP3191a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP3191a, RRID:AB_637729
- Product name
- Phospho-p27Kip1(S10) Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Androgen Receptor Enhances p27 Degradation in Prostate Cancer Cells through Rapid and Selective TORC2 Activation.
Gefitinib resistance of cancer cells correlated with TM4SF5-mediated epithelial-mesenchymal transition.
The role of p27(Kip1) in dasatinib-enhanced paclitaxel cytotoxicity in human ovarian cancer cells.
Fang Z, Zhang T, Dizeyi N, Chen S, Wang H, Swanson KD, Cai C, Balk SP, Yuan X
The Journal of biological chemistry 2012 Jan 13;287(3):2090-8
The Journal of biological chemistry 2012 Jan 13;287(3):2090-8
Gefitinib resistance of cancer cells correlated with TM4SF5-mediated epithelial-mesenchymal transition.
Lee MS, Kim HP, Kim TY, Lee JW
Biochimica et biophysica acta 2012 Feb;1823(2):514-23
Biochimica et biophysica acta 2012 Feb;1823(2):514-23
The role of p27(Kip1) in dasatinib-enhanced paclitaxel cytotoxicity in human ovarian cancer cells.
Le XF, Mao W, He G, Claret FX, Xia W, Ahmed AA, Hung MC, Siddik ZH, Bast RC Jr
Journal of the National Cancer Institute 2011 Sep 21;103(18):1403-22
Journal of the National Cancer Institute 2011 Sep 21;103(18):1403-22
No comments: Submit comment
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- The anti-Phospho-p27Kip1-S10 Pab (Cat. #AP9191a) is used in Western blot to detect Phospho-p27Kip1-S10 in HL60 tissue lysate
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human skeletal muscle with Phospho-p27Kip1-S10 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:50~100
