LS-C354630

antibody from LSBio

Targeting: CDKN1B KIP1, P27KIP1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C354630

Provider

LSBio

Product name

CDKN1B / p27 Kip1 Antibody (phospho-Thr187) LS-C354630

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse, Bovine, Canine

Host

Rabbit

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical analysis of p27 Kip1 (pT187) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemical analysis of p27 Kip1 (pT187) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w