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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- GTX120754 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX120754, RRID:AB_11165789
- Product name
- CCDC14 antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.
Proximity interactions among centrosome components identify regulators of centriole duplication.
Kodani A, Yu TW, Johnson JR, Jayaraman D, Johnson TL, Al-Gazali L, Sztriha L, Partlow JN, Kim H, Krup AL, Dammermann A, Krogan NJ, Walsh CA, Reiter JF
eLife 2015 Aug 22;4
eLife 2015 Aug 22;4
Proximity interactions among centrosome components identify regulators of centriole duplication.
Firat-Karalar EN, Rauniyar N, Yates JR 3rd, Stearns T
Current biology : CB 2014 Mar 17;24(6):664-70
Current biology : CB 2014 Mar 17;24(6):664-70
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: A431 7.5% SDS PAGE GTX120754 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CCDC14 antibody [C2C3], C-term (GTX120754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A431, using CCDC14(GTX120754) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CCDC14 antibody [C2C3], C-term detects CCDC14 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CCDC14 protein stained by CCDC14 antibody [C2C3], C-term (GTX120754) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.
