PAB20286

antibody from Abnova Corporation

Targeting: ZMAT5 SNRNP20, ZC3H19

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20286

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20286, RRID:AB_10987170

Product name

ZMAT5 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ZMAT5.

Antigen sequence

RKKHLNGLQHLKAKKVWYDMFRDAAAILLDEQNKR
PCRKFLLTGQCDFGSNCRFSHMSERDLQELSIQVE
EERRAREWLLDAPELPEGHLEDWLEKRAKRLSSAP
S


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with ZMAT5 polyclonal antibody (Cat # PAB20286) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with ZMAT5 polyclonal antibody (Cat # PAB20286) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node with ZMAT5 polyclonal antibody (Cat # PAB20286) shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)