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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00002965-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00002965-M01, RRID:AB_425470
- Product name
- GTF2H1 monoclonal antibody (M01), clone 1F12-1B5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant GTF2H1.
- Antigen sequence
MATSSEEVLLIVKKVRQKKQDGALYLMAERIAWAP
EGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLH
AGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKR
KANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAE
EFWANRLNVNATDSSSTSNHKQDVGISAAFLADVR
PQTDGCNGLRYNLTSDIIESIFRTYPAVKMKYAEN
VPHNMTEKEFWTRFFQSHYFHRDRLNTGSKDLFAE
CAKIDEKGLKTMVSLGVKNPLLDLTALEDKPLDEG
YGISSVPSASNSKSIKENSNAAIIKRFNHHSAMVL
AAGLRKQEAQNEQTSEPSNMDGNSGDADCFQPAVK
RAKLQESIEYEDLGKNNSVKTIALNLKKSDRYYHG
PTPIQSLQYATSQDIINSFQSIRQEMEAYTPKLTQ
VLSSSAASSTITALSPGGALMQGGTQQAINQMVPN
DIQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKV
VKMKSNLERFQVTKLCPFQEKIRRQYLSTNLVSHI
EEMLQTAYNKLHTWQSRRLMKKT- Isotype
- IgG
- Antibody clone number
- 1F12-1B5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references XPB induces C1D expression to counteract UV-induced apoptosis.
Li G, Liu J, Abu-Asab M, Masabumi S, Maru Y
Molecular cancer research : MCR 2010 Jun;8(6):885-95
Molecular cancer research : MCR 2010 Jun;8(6):885-95
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 Western Blot analysis of GTF2H1 expression in Jurkat ( Cat # L017V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- GTF2H1 monoclonal antibody (M01), clone 1F12-1B5. Western Blot analysis of GTF2H1 expression in Hela S3 NE ( Cat # L013V3 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody (M01), clone 1F12-1B5.Lane 1: GTF2H1 transfected lysate(62 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged GTF2H1 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to GTF2H1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue.[antibody concentration 5 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
