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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00002965-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002965-M01, RRID:AB_539384
- Product name
- Mouse Monoclonal GTF2H1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. GTF2H1 - general transcription factor IIH, polypeptide 1, 62kDa
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Interrogation of nucleotide excision repair capacity: impact on platinum-based cancer therapy.
XPB induces C1D expression to counteract UV-induced apoptosis.
Earley JN, Turchi JJ
Antioxidants & redox signaling 2011 Jun 15;14(12):2465-77
Antioxidants & redox signaling 2011 Jun 15;14(12):2465-77
XPB induces C1D expression to counteract UV-induced apoptosis.
Li G, Liu J, Abu-Asab M, Masabumi S, Maru Y
Molecular cancer research : MCR 2010 Jun;8(6):885-95
Molecular cancer research : MCR 2010 Jun;8(6):885-95
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 over-expressed 293 cell line, cotransfected with GTF2H1 Validated Chimera RNAi ( Cat # H00002965-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 (Cat # H00002965-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody (M01), clone 1F12-1B5.Lane 1: GTF2H1 transfected lysate(62 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - GTF2H1 monoclonal antibody (M01), clone 1F12-1B5. Analysis of GTF2H1 expression in Hela S3 NE.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 Analysis of GTF2H1 expression in Jurkat.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Detection limit for recombinant GST tagged GTF2H1 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue.Antibody concentration 5 ug/ml.
