Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 14293-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FN3K Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MEQLLRAEL RTATLRAFGG PGAGCISEGR AYDTDAGPVF VKVNRRTQAR QMFEGEVASL EALRSTGLVR VPRPMKVIDL PGGGAAFVME HLKMKSLSSQ ASKLGEQMAD LHLYNQKLRE KLKEEENTVG RRGEGAEPQY VDKFGFHTVT CCGFIPQVNE WQDDWPTFFA RHRLQAQLDL IEKDYADREA RELWSRLQVK IPDLFCGLEI VPALLHGDLW SGNVAEDDVG PIIYDPASFY GHSEFELAIA LMFGGFPRSF FTAYHRKIPK APGFDQRLLL YQLFNYLNHW NHFGREYRSP SLGTMRRLLK (1-309 aa encoded by BC042680)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.19 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human kidney tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human heart tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 14293-1-AP (FN3K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 14293-1-AP (FN3K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14293-1-AP (FN3K antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14293-1-AP (FN3K antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-FN3K (IP:14293-1-AP, 3ug; Detection:14293-1-AP 1:500) with HeLa cells lysate 1840ug.