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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-28603 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FN3K Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2.
- Concentration
- 0.94 mg/mL
Submitted references The Oncogenic Action of NRF2 Depends on De-glycation by Fructosamine-3-Kinase.
Sanghvi VR, Leibold J, Mina M, Mohan P, Berishaj M, Li Z, Miele MM, Lailler N, Zhao C, de Stanchina E, Viale A, Akkari L, Lowe SW, Ciriello G, Hendrickson RC, Wendel HG
Cell 2019 Aug 8;178(4):807-819.e21
Cell 2019 Aug 8;178(4):807-819.e21
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FN3K using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a FN3K polyclonal antibody (Product # PA5-28603) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FN3K Polyclonal Antibody (Product # PA5-28603). Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with FN3K Polyclonal Antibody (Product # PA5-28603) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using FN3K (Product # PA5-28603) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
