Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-31372 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HECA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa.
- Concentration
- 0.7 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HECA Polyclonal Antibody (Product # PA5-31372). Sample (30 µg of whole cell lysate). Lane A: A549 . 7.5% SDS PAGE. HECA Polyclonal Antibody (Product # PA5-31372) diluted at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HECA in methanol-fixed HeLa cells using a HECA polyclonal antibody (Product # PA5-31372) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HECA Polyclonal Antibody (Product # PA5-31372) HECA protein at cytoplasm on human adrenal gland tumor blood vessel smooth muscle cell by immunohistochemical analysis. Sample: Paraffin-embedded adrenal gland tumor blood vessel smooth muscle cell. HECA Polyclonal Antibody. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.