Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- 20772-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#20772-1-AP, RRID:AB_10695765
- Product name
- LIMS1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated LIMS1 antibody (Cat. #20772-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.
PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.
A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.
Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.
Cui C, Wang J, Guo L, Wu C
Amino acids 2021 Dec;53(12):1875-1890
Amino acids 2021 Dec;53(12):1875-1890
PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.
Guo L, Cui C, Wang J, Yuan J, Yang Q, Zhang P, Su W, Bao R, Ran J, Wu C
Nature communications 2020 Oct 1;11(1):4913
Nature communications 2020 Oct 1;11(1):4913
A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.
Guo L, Wang R, Zhang K, Yuan J, Wang J, Wang X, Ma J, Wu C
The Journal of cell biology 2019 Nov 4;218(11):3773-3794
The Journal of cell biology 2019 Nov 4;218(11):3773-3794
Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.
Huet-Calderwood C, Brahme NN, Kumar N, Stiegler AL, Raghavan S, Boggon TJ, Calderwood DA
Journal of cell science 2014 Oct 1;127(Pt 19):4308-21
Journal of cell science 2014 Oct 1;127(Pt 19):4308-21
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human kidney tissue were subjected to SDS PAGE followed by western blot with 20772-1-AP(LIMS1 antibody) at dilution of 1:400
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using LIMS1 antibody 20772-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human oesophagus cancer using 20772-1-AP(LIMS1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human oesophagus cancer using 20772-1-AP(LIMS1 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The LIMS1 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human LIMS1. This antibody recognizes human,mouse,rat antigen. The LIMS1 antibody has been validated for the following applications: ELISA, WB, IF, IHC, FC analysis.