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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB6257 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal PINCH1/LIMS1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat PINCH1 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references PINCH proteins regulate cardiac contractility by modulating integrin-linked kinase-protein kinase B signaling.
Meder B, Huttner IG, Sedaghat-Hamedani F, Just S, Dahme T, Frese KS, Vogel B, Köhler D, Kloos W, Rudloff J, Marquart S, Katus HA, Rottbauer W
Molecular and cellular biology 2011 Aug;31(16):3424-35
Molecular and cellular biology 2011 Aug;31(16):3424-35
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Detection of Human and Rat PINCH1 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, WI-38 human lung fibroblast cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PINCH1 Monoclonal Antibody (Catalog # MAB6257) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PINCH1 at approximately 37-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
