Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB10297 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10297, RRID:AB_11188576
- Product name
- LIMS1 monoclonal antibody, clone 5G7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant LIMS1.
- Isotype
- IgG
- Antibody clone number
- 5G7
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using LIMS1 monoclonal antobody, clone 5G7 (Cat # MAB10297) against A-549 (1), Jurkat (2), and HeLa (3) cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using LIMS1 monoclonal antobody, clone 5G7 (Cat # MAB10297) against recombinant human LIMS1 protein.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HepG2 cells using LIMS1 monoclonal antobody, clone 5G7 (Cat # MAB10297) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using LIMS1 monoclonal antobody, clone 5G7 (Cat # MAB10297) (blue) and negative control (red).
- Validation comment
- Flow Cytometry