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Learn20772-1-AP
antibody from Invitrogen Antibodies
Targeting: LIMS1
PINCH, PINCH1
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 20772-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LIMS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MANALASAT CERCKGGFAP AEKIVNSNGE LYHEQCFVCA QCFQQFPEGL FYEFEGRKYC EHDFQMLFAP CCHQCGEFTI GRVIKAMNNS WHPECFRCDL CQEVLADIGF VKNAGRHLCR PCHNREKARG LGKYICQKCH AIIDEQPLIF KNDPYHPDHF NCANCGKELT ADARELKGEL YCLPCHDKMG VPICGACRRP IEGRVVNAMG KQWHVEHFVC AKCEKPFLGH RHYERKGLAY CETHYNQLFG DVCFHCNRVI EGGVVSALNK AWCVNCFACS TCNTKLTLKN KFVEFDMKPV CKKCYEKFPL ELKKRLKKLA ETLGRK (1-325 aa encoded by B C005341)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.21 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Caco-2 cells were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of LIMS1 antibody (20772-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIMS1 transfected HeLa cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human kidney tissue were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using LIMS1 antibody 20772-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human oesophagus cancer using 20772-1-AP (LIMS1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human oesophagus cancer using 20772-1-AP (LIMS1 antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug LIMS1 antibody (20772-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-LIMS1 (IP:20772-1-AP, 4ug; Detection:20772-1-AP 1:500) with K-562 cells lysate 3600ug.
