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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB1701 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1701, RRID:AB_1238150
- Product name
- UBE2S polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of UBE2S.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Efficient APC/C substrate degradation in cells undergoing mitotic exit depends on K11 ubiquitin linkages.
UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit.
Min M, Mevissen TE, De Luca M, Komander D, Lindon C
Molecular biology of the cell 2015 Dec 1;26(24):4325-32
Molecular biology of the cell 2015 Dec 1;26(24):4325-32
UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit.
Garnett MJ, Mansfeld J, Godwin C, Matsusaka T, Wu J, Russell P, Pines J, Venkitaraman AR
Nature cell biology 2009 Nov;11(11):1363-9
Nature cell biology 2009 Nov;11(11):1363-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- UBE2S polyclonal antibody (Cat # PAB1701) is used in Western blot to detect UBE2S in Jurkat cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of UBE2S (arrow) using UBE2S polyclonal antibody (Cat # PAB1701). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2S gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBE2S polyclonal antibody (Cat # PAB1701) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
