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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA1-082 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RANBP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-082 detects Ran binding protein 2 (RanBP2) from human and bovine retina.
- Concentration
- 1 mg/mL
Submitted references Species-specific vulnerability of RanBP2 shaped the evolution of SIV as it transmitted in African apes.
Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis.
Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity.
Meyerson NR, Warren CJ, Vieira DASA, Diaz-Griferro F, Sawyer SL
PLoS pathogens 2018 Mar;14(3):e1006906
PLoS pathogens 2018 Mar;14(3):e1006906
Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis.
Asally M, Yasuda Y, Oka M, Otsuka S, Yoshimura SH, Takeyasu K, Yoneda Y
The FEBS journal 2011 Feb;278(4):610-21
The FEBS journal 2011 Feb;278(4):610-21
Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity.
Hamard PJ, Boyer-Guittaut M, Camuzeaux B, Dujardin D, Hauss C, Oelgeschläger T, Vigneron M, Kedinger C, Chatton B
Nucleic acids research 2007;35(4):1134-44
Nucleic acids research 2007;35(4):1134-44
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of RanBP2 from human retinal extracts using Product # PA1-082.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of RanBP2 from human retinal extracts using Product # PA1-082.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RanBP2 in bovine retina using Product # PA1-082.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 7 HIV-1, SIVcpz, and SIVgor are equally dependent on RanBP2. A) 293T cells were infected with lentivirus harboring the indicated shRNA constructs and MTT-proliferation assays were carried out in triplicate at 96 hours post infection. Absorbance was measured at a wavelength of 590 nm. Reduced absorbance is indicative of reduced cell proliferation. B) Whole cell extract (WCE) from 293T cells infected with lentivirus harboring the indicated shRNA construct was collected at 72 hours post infection and immunoblotted with antibodies raised against RanBP2 or Nup153 as a loading control. C) 293T cells treated with the indicated shRNA constructs were infected with HIV-1 bearing the indicated cyclophilin-binding loop (X-axis) and a GFP reporter. The percentage cells infected in each sample was normalized to the shRNA control. Infections were performed in triplicate and error bars represent twice the standard error of the mean.
