Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00001656-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001656-M01, RRID:AB_538221
- Product name
- Mouse Monoclonal DDX6 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. DDX6 - DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references DDX6 localizes to nuage structures and the annulus of mammalian spermatogenic cells.
The DEAD-box RNA helicase DDX6 is required for efficient encapsidation of a retroviral genome.
Rck/p54 interacts with APP mRNA as part of a multi-protein complex and enhances APP mRNA and protein expression in neuronal cell lines.
Kawahara C, Yokota S, Fujita H
Histochemistry and cell biology 2014 Jan;141(1):111-21
Histochemistry and cell biology 2014 Jan;141(1):111-21
The DEAD-box RNA helicase DDX6 is required for efficient encapsidation of a retroviral genome.
Yu SF, Lujan P, Jackson DL, Emerman M, Linial ML
PLoS pathogens 2011 Oct;7(10):e1002303
PLoS pathogens 2011 Oct;7(10):e1002303
Rck/p54 interacts with APP mRNA as part of a multi-protein complex and enhances APP mRNA and protein expression in neuronal cell lines.
Broytman O, Westmark PR, Gurel Z, Malter JS
Neurobiology of aging 2009 Dec;30(12):1962-74
Neurobiology of aging 2009 Dec;30(12):1962-74
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX6 Antibody (3D2) [H00001656-M01] - Analysis of DDX6 over-expressed 293 cell line, cotransfected with DDX6 Validated Chimera RNAi ( Cat # H00001656-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DDX6 monoclonal antibody (M01), clone 3D2 (Cat # H00001656-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX6 Antibody (3D2) [H00001656-M01] - Analysis of DDX6 expression in transfected 293T cell line by DDX6 monoclonal antibody (M01), clone 3D2.Lane 1: DDX6 transfected lysate(53.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX6 Antibody (3D2) [H00001656-M01] - DDX6 monoclonal antibody (M01), clone 3D2 Analysis of DDX6 expression in Hela.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DDX6 Antibody (3D2) [H00001656-M01] - Analysis of monoclonal antibody to DDX6 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.