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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NB200-192 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-192, RRID:AB_10000566
- Product name
- Rabbit Polyclonal DDX6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Alteration of SC35 localization by transfection reagents.
HNRNPK maintains epidermal progenitor function through transcription of proliferation genes and degrading differentiation promoting mRNAs.
The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis.
5-Fluorouracil affects assembly of stress granules based on RNA incorporation.
A monoclonal antibody against p53 cross-reacts with processing bodies.
Ataxin-2-like is a regulator of stress granules and processing bodies.
Evasion of the innate immune response: the Old World alphavirus nsP2 protein induces rapid degradation of Rpb1, a catalytic subunit of RNA polymerase II.
FUSE binding protein 1 interacts with untranslated regions of Japanese encephalitis virus RNA and negatively regulates viral replication.
Competition for XPO5 binding between Dicer mRNA, pre-miRNA and viral RNA regulates human Dicer levels.
Mitochondria associate with P-bodies and modulate microRNA-mediated RNA interference.
Role of p54 RNA helicase activity and its C-terminal domain in translational repression, P-body localization and assembly.
The virion-packaged endoribonuclease of herpes simplex virus 1 cleaves mRNA in polyribosomes.
Nucleocytoplasmic traffic of CPEB1 and accumulation in Crm1 nucleolar bodies.
Ribonomic analysis of human Pum1 reveals cis-trans conservation across species despite evolution of diverse mRNA target sets.
Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells.
Human immunodeficiency virus type 1 Vif functionally interacts with diverse APOBEC3 cytidine deaminases and moves with them between cytoplasmic sites of mRNA metabolism.
Coordinated changes in mRNA turnover, translation, and RNA processing bodies in bronchial epithelial cells following inflammatory stimulation.
Ribosomal slowdown mediates translational arrest during cellular division.
3' Terminal oligo U-tract-mediated stimulation of decapping.
Damodaran AP, Courthéoux T, Watrin E, Prigent C
Biochimica et biophysica acta. Molecular cell research 2020 Apr;1867(4):118650
Biochimica et biophysica acta. Molecular cell research 2020 Apr;1867(4):118650
HNRNPK maintains epidermal progenitor function through transcription of proliferation genes and degrading differentiation promoting mRNAs.
Li J, Chen Y, Xu X, Jones J, Tiwari M, Ling J, Wang Y, Harismendy O, Sen GL
Nature communications 2019 Sep 13;10(1):4198
Nature communications 2019 Sep 13;10(1):4198
The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis.
Di Stefano B, Luo EC, Haggerty C, Aigner S, Charlton J, Brumbaugh J, Ji F, Rabano Jiménez I, Clowers KJ, Huebner AJ, Clement K, Lipchina I, de Kort MAC, Anselmo A, Pulice J, Gerli MFM, Gu H, Gygi SP, Sadreyev RI, Meissner A, Yeo GW, Hochedlinger K
Cell stem cell 2019 Nov 7;25(5):622-638.e13
Cell stem cell 2019 Nov 7;25(5):622-638.e13
5-Fluorouracil affects assembly of stress granules based on RNA incorporation.
Kaehler C, Isensee J, Hucho T, Lehrach H, Krobitsch S
Nucleic acids research 2014 Jun;42(10):6436-47
Nucleic acids research 2014 Jun;42(10):6436-47
A monoclonal antibody against p53 cross-reacts with processing bodies.
Thomas MG, Luchelli L, Pascual M, Gottifredi V, Boccaccio GL
PloS one 2012;7(5):e36447
PloS one 2012;7(5):e36447
Ataxin-2-like is a regulator of stress granules and processing bodies.
Kaehler C, Isensee J, Nonhoff U, Terrey M, Hucho T, Lehrach H, Krobitsch S
PloS one 2012;7(11):e50134
PloS one 2012;7(11):e50134
Evasion of the innate immune response: the Old World alphavirus nsP2 protein induces rapid degradation of Rpb1, a catalytic subunit of RNA polymerase II.
Akhrymuk I, Kulemzin SV, Frolova EI
Journal of virology 2012 Jul;86(13):7180-91
Journal of virology 2012 Jul;86(13):7180-91
FUSE binding protein 1 interacts with untranslated regions of Japanese encephalitis virus RNA and negatively regulates viral replication.
Chien HL, Liao CL, Lin YL
Journal of virology 2011 May;85(10):4698-706
Journal of virology 2011 May;85(10):4698-706
Competition for XPO5 binding between Dicer mRNA, pre-miRNA and viral RNA regulates human Dicer levels.
Bennasser Y, Chable-Bessia C, Triboulet R, Gibbings D, Gwizdek C, Dargemont C, Kremer EJ, Voinnet O, Benkirane M
Nature structural & molecular biology 2011 Mar;18(3):323-7
Nature structural & molecular biology 2011 Mar;18(3):323-7
Mitochondria associate with P-bodies and modulate microRNA-mediated RNA interference.
Huang L, Mollet S, Souquere S, Le Roy F, Ernoult-Lange M, Pierron G, Dautry F, Weil D
The Journal of biological chemistry 2011 Jul 8;286(27):24219-30
The Journal of biological chemistry 2011 Jul 8;286(27):24219-30
Role of p54 RNA helicase activity and its C-terminal domain in translational repression, P-body localization and assembly.
Minshall N, Kress M, Weil D, Standart N
Molecular biology of the cell 2009 May;20(9):2464-72
Molecular biology of the cell 2009 May;20(9):2464-72
The virion-packaged endoribonuclease of herpes simplex virus 1 cleaves mRNA in polyribosomes.
Taddeo B, Zhang W, Roizman B
Proceedings of the National Academy of Sciences of the United States of America 2009 Jul 21;106(29):12139-44
Proceedings of the National Academy of Sciences of the United States of America 2009 Jul 21;106(29):12139-44
Nucleocytoplasmic traffic of CPEB1 and accumulation in Crm1 nucleolar bodies.
Ernoult-Lange M, Wilczynska A, Harper M, Aigueperse C, Dautry F, Kress M, Weil D
Molecular biology of the cell 2009 Jan;20(1):176-87
Molecular biology of the cell 2009 Jan;20(1):176-87
Ribonomic analysis of human Pum1 reveals cis-trans conservation across species despite evolution of diverse mRNA target sets.
Morris AR, Mukherjee N, Keene JD
Molecular and cellular biology 2008 Jun;28(12):4093-103
Molecular and cellular biology 2008 Jun;28(12):4093-103
Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells.
Zheng D, Ezzeddine N, Chen CY, Zhu W, He X, Shyu AB
The Journal of cell biology 2008 Jul 14;182(1):89-101
The Journal of cell biology 2008 Jul 14;182(1):89-101
Human immunodeficiency virus type 1 Vif functionally interacts with diverse APOBEC3 cytidine deaminases and moves with them between cytoplasmic sites of mRNA metabolism.
Marin M, Golem S, Rose KM, Kozak SL, Kabat D
Journal of virology 2008 Jan;82(2):987-98
Journal of virology 2008 Jan;82(2):987-98
Coordinated changes in mRNA turnover, translation, and RNA processing bodies in bronchial epithelial cells following inflammatory stimulation.
Zhai Y, Zhong Z, Chen CY, Xia Z, Song L, Blackburn MR, Shyu AB
Molecular and cellular biology 2008 Dec;28(24):7414-26
Molecular and cellular biology 2008 Dec;28(24):7414-26
Ribosomal slowdown mediates translational arrest during cellular division.
Sivan G, Kedersha N, Elroy-Stein O
Molecular and cellular biology 2007 Oct;27(19):6639-46
Molecular and cellular biology 2007 Oct;27(19):6639-46
3' Terminal oligo U-tract-mediated stimulation of decapping.
Song MG, Kiledjian M
RNA (New York, N.Y.) 2007 Dec;13(12):2356-65
RNA (New York, N.Y.) 2007 Dec;13(12):2356-65
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX6 Antibody [NB200-192] - Human DDX6 on HeLa whole cell lysate using NB200-192. DDX6 was also IPed using rabbit anti-DDX6 antibody NB200-191, but not with 'DDX6 Ab', which is to another epitope from DDX6. Normal rabbit IgG was used as a negative control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX6 Antibody [NB200-192] - Whole cell lysate (15 and 50 ug) from NIH3T3 cells. Antibody used at 0.2 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX6 Antibody [NB200-192] - Detection of Human and Mouse DDX6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX6 antibody NB200-192 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-192] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DDX6 (left) and Lot 3 (right) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
