Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00151011-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00151011-M01, RRID:AB_426137
- Product name
- SEPT10 monoclonal antibody (M01), clone 2A12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant SEPT10.
- Antigen sequence
MASSEVARHLLFQSHMATKTTCMSSQGSDDEQIKR
ENIRSLTMSGHVGFESLPDQLVNRSIQQGFCFNIL
CVGETGIGKSTLIDTLFNTNFEDYESSHFCPNVKL
KAQTYELQESNVQLKLTIVNTVGFGDQINKEESYQ
PIVDYIDAQFEAYLQEELKIKRSLFTYHDSRIHVC
LYFISPTGHSLKTLDLLTMKNLDSKVNIIPVIAKA
DTVSKTELQKFKIKLMSELVSNGVQIYQFPTDDDT
IAKVNAAMNGQLPFAVVGSMDEVKVGNKMVKARQY
PWGVVQVENENHCDFVKLREMLICTNMEDLREQTH
TRHYELYRRCKLEEMGFTDVGPENKPVSVQETYEA
KRHEFHGERQRKEEEMKQMFVQRVKEKEAILKEAE
RELQAKFEHLKRLHQEERMKLEEKRRLLEEEIIAF
SKKKATSEIFHSQSFLATGSNLRKDKDRKNSNFL- Isotype
- IgG
- Antibody clone number
- 2A12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Identification of a novel role of Septin 10 in paclitaxel-resistance in cancers through a functional genomics screen.
Xu M, Takanashi M, Oikawa K, Nishi H, Isaka K, Yoshimoto T, Ohyashiki J, Kuroda M
Cancer science 2012 Apr;103(4):821-7
Cancer science 2012 Apr;103(4):821-7
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SEPT10 monoclonal antibody (M01), clone 2A12 Western Blot analysis of SEPT10 expression in U-2 OS ( Cat # L022V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SEPT10 expression in transfected 293T cell line by SEPT10 monoclonal antibody (M01), clone 2A12.Lane 1: SEPT10 transfected lysate(52.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SEPT10 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to SEPT10 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
