MA5-23101

antibody from Invitrogen Antibodies

Targeting: SEPTIN10 FLJ11619, SEPT10

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23101

Provider

Invitrogen Antibodies

Product name

Septin-10 Monoclonal Antibody (2A12)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Peptide Sequence: MASSEVARHL LFQSHMATKT TCMSSQGSDD EQIKRENIRS LTMSGHVGFE SLPDQLVNRS IQQGFCFNIL CVGETGIGKS TLIDTLFNTN FEDYESSHFC PNVKLKAQTY ELQESNVQLK LTIVNTVGFG DQINKEESYQ PIVDYIDAQF EAYLQEELKI KRSLFTYHDS RIHVCLYFIS PTGHSLKTLD LLTMKNLDSK VNIIPVIAKA DTVSKTELQK FKIKLMSELV SNGVQIYQFP TDDDTIAKVN AAMNGQLPFA VVGSMDEVKV GNKMVKARQY PWGVVQVENE NHCDFVKLRE MLICTNMEDL REQTHTRHYE LYRRCKLEEM GFTDVGPENK PVSVQETYEA KRHEFHGERQ RKEEEMKQMF VQRVKEKEAI LKEAERELQA KFEHLKRLHQ EERMKLEEKR RLLEEEIIAF SKKKATSEIF HSQSFLATGS NLRKDKDRKN SNFL

Antibody clone number

2A12

Concentration

1.0 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SEPT10 expressed in transfected 293T cell line by anti-SEPT10 monoclonal antibody (Product # MA5-23101), clone 2A12. Lane 1: SEPT10 transfected lysate (52.6 KDa). Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SEPT10 in cell lysate from U-2 OS cells, using a anti-SEPT10 monoclonal antibody (Product # MA5-23101).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SEPT10 expressed in transfected 293T cell line by anti-SEPT10 monoclonal antibody (Product # MA5-23101), clone 2A12. Lane 1: SEPT10 transfected lysate (52.6 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using an anti-SEPT10 monoclonal antibody (Product # MA5-23101). (antibody concentration 10 µg/mL).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SEPT10 using anti-SEPT10 monoclonal antibody (Product # MA5-23101) is approximately 1 ng/mL as a capture antibody.