GTX119102

antibody from GeneTex

Targeting: UBE3C KIAA0010, KIAA10

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX119102

Provider

GeneTex

Proper citation

GeneTex Cat#GTX119102, RRID:AB_10617623

Product name

UBE3C antibody [N1N3]

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Autoubiquitination of the 26S proteasome on Rpn13 regulates breakdown of ubiquitin conjugates.

Besche HC, Sha Z, Kukushkin NV, Peth A, Hock EM, Kim W, Gygi S, Gutierrez JA, Liao H, Dick L, Goldberg AL
The EMBO journal 2014 May 16;33(10):1159-76

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The E3 ubiquitin ligase UBE3C enhances proteasome processivity by ubiquitinating partially proteolyzed substrates.

Chu BW, Kovary KM, Guillaume J, Chen LC, Teruel MN, Wandless TJ
The Journal of biological chemistry 2013 Nov 29;288(48):34575-87

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Identification and proteomic analysis of distinct UBE3A/E6AP protein complexes.

Martínez-Noël G, Galligan JT, Sowa ME, Arndt V, Overton TM, Harper JW, Howley PM
Molecular and cellular biology 2012 Aug;32(15):3095-106

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ug of whole cell lysate) A: mouse brain 5% SDS PAGE GTX119102 diluted at 1:500

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE GTX119102 diluted at 1:1000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UBE3C(GTX119102) antibody at 1:500 dilution.