MAB1770

antibody from Abnova Corporation

Targeting: ACOT11 BFIT, BFIT1, KIAA0707, STARD14, THEA, THEM1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1770

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB1770, RRID:AB_1671819

Product name

ACOT11 monoclonal antibody, clone J4B2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against partial recombinant ACOT11.

Isotype

IgG

Antibody clone number

J4B2

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

BFIT, a unique acyl-CoA thioesterase induced in thermogenic brown adipose tissue: cloning, organization of the human gene and assessment of a potential link to obesity.

Adams SH, Chui C, Schilbach SL, Yu XX, Goddard AD, Grimaldi JC, Lee J, Dowd P, Colman S, Lewin DA
The Biochemical journal 2001 Nov 15;360(Pt 1):135-42

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Cell lysates of LNCap (lane 1), Hep3B (lane 2) (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with ACOT11 monoclonal antibody, clone J4B2 (Cat # MAB1770) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Paraffin embedded sections of human colon cancer tissue were incubated with ACOT11 monoclonal antibody, clone J4B2 (Cat # MAB1770) (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)