Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB300-959 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-959, RRID:AB_2283399
- Product name
- Goat Polyclonal Proprotein Convertase 9/PCSK9 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Proprotein Convertase Subtilisin/Kexin Type 9 Is Associated with Degenerating Adipocytes in Abdominal Aortic Aneurysm.
Loss-of-function PCSK9 mutants evade the unfolded protein response sensor GRP78 and fail to induce endoplasmic reticulum stress when retained.
Endoplasmic Reticulum Stress and Ca2+ Depletion Differentially Modulate the Sterol Regulatory Protein PCSK9 to Control Lipid Metabolism.
Modulation of cholesterol transport by insulin-treated gestational diabetes mellitus in human full-term placenta.
The NADPH oxidase subunit NOX4 is a new target gene of the hypoxia-inducible factor-1.
Expression of the hepatic Niemann-Pick C1 like 1 protein gene is sensitive to rosuvastatin treatment of primary human hepatocytes.
A new PCSK9 gene promoter variant affects gene expression and causes autosomal dominant hypercholesterolemia.
Plasma PCSK9 concentrations correlate with LDL and total cholesterol in diabetic patients and are decreased by fenofibrate treatment.
PCSK9 is phosphorylated by a Golgi casein kinase-like kinase ex vivo and circulates as a phosphoprotein in humans.
The proprotein convertase (PC) PCSK9 is inactivated by furin and/or PC5/6A: functional consequences of natural mutations and post-translational modifications.
Evidence for effect of mutant PCSK9 on apolipoprotein B secretion as the cause of unusually severe dominant hypercholesterolaemia.
Low LDL cholesterol in individuals of African descent resulting from frequent nonsense mutations in PCSK9.
Tanaka H, Inuzuka K, Iida Y, Shimizu H, Unno N, Urano T
Journal of oleo science 2018 Oct 11;67(10):1355-1360
Journal of oleo science 2018 Oct 11;67(10):1355-1360
Loss-of-function PCSK9 mutants evade the unfolded protein response sensor GRP78 and fail to induce endoplasmic reticulum stress when retained.
Lebeau P, Platko K, Al-Hashimi AA, Byun JH, Lhoták Š, Holzapfel N, Gyulay G, Igdoura SA, Cool DR, Trigatti B, Seidah NG, Austin RC
The Journal of biological chemistry 2018 May 11;293(19):7329-7343
The Journal of biological chemistry 2018 May 11;293(19):7329-7343
Endoplasmic Reticulum Stress and Ca2+ Depletion Differentially Modulate the Sterol Regulatory Protein PCSK9 to Control Lipid Metabolism.
Lebeau P, Al-Hashimi A, Sood S, Lhoták Š, Yu P, Gyulay G, Paré G, Chen SR, Trigatti B, Prat A, Seidah NG, Austin RC
The Journal of biological chemistry 2017 Jan 27;292(4):1510-1523
The Journal of biological chemistry 2017 Jan 27;292(4):1510-1523
Modulation of cholesterol transport by insulin-treated gestational diabetes mellitus in human full-term placenta.
Dubé E, Ethier-Chiasson M, Lafond J
Biology of reproduction 2013 Jan;88(1):16
Biology of reproduction 2013 Jan;88(1):16
The NADPH oxidase subunit NOX4 is a new target gene of the hypoxia-inducible factor-1.
Diebold I, Petry A, Hess J, Görlach A
Molecular biology of the cell 2010 Jun 15;21(12):2087-96
Molecular biology of the cell 2010 Jun 15;21(12):2087-96
Expression of the hepatic Niemann-Pick C1 like 1 protein gene is sensitive to rosuvastatin treatment of primary human hepatocytes.
Brulhart-Meynet MC, Deakin SP, Sgroi A, Buhler L, James RW
Pharmacogenetics and genomics 2010 Jul;20(7):455-8
Pharmacogenetics and genomics 2010 Jul;20(7):455-8
A new PCSK9 gene promoter variant affects gene expression and causes autosomal dominant hypercholesterolemia.
Blesa S, Vernia S, Garcia-Garcia AB, Martinez-Hervas S, Ivorra C, Gonzalez-Albert V, Ascaso JF, Martín-Escudero JC, Real JT, Carmena R, Casado M, Chaves FJ
The Journal of clinical endocrinology and metabolism 2008 Sep;93(9):3577-83
The Journal of clinical endocrinology and metabolism 2008 Sep;93(9):3577-83
Plasma PCSK9 concentrations correlate with LDL and total cholesterol in diabetic patients and are decreased by fenofibrate treatment.
Lambert G, Ancellin N, Charlton F, Comas D, Pilot J, Keech A, Patel S, Sullivan DR, Cohn JS, Rye KA, Barter PJ
Clinical chemistry 2008 Jun;54(6):1038-45
Clinical chemistry 2008 Jun;54(6):1038-45
PCSK9 is phosphorylated by a Golgi casein kinase-like kinase ex vivo and circulates as a phosphoprotein in humans.
Dewpura T, Raymond A, Hamelin J, Seidah NG, Mbikay M, Chrétien M, Mayne J
The FEBS journal 2008 Jul;275(13):3480-93
The FEBS journal 2008 Jul;275(13):3480-93
The proprotein convertase (PC) PCSK9 is inactivated by furin and/or PC5/6A: functional consequences of natural mutations and post-translational modifications.
Benjannet S, Rhainds D, Hamelin J, Nassoury N, Seidah NG
The Journal of biological chemistry 2006 Oct 13;281(41):30561-72
The Journal of biological chemistry 2006 Oct 13;281(41):30561-72
Evidence for effect of mutant PCSK9 on apolipoprotein B secretion as the cause of unusually severe dominant hypercholesterolaemia.
Sun XM, Eden ER, Tosi I, Neuwirth CK, Wile D, Naoumova RP, Soutar AK
Human molecular genetics 2005 May 1;14(9):1161-9
Human molecular genetics 2005 May 1;14(9):1161-9
Low LDL cholesterol in individuals of African descent resulting from frequent nonsense mutations in PCSK9.
Cohen J, Pertsemlidis A, Kotowski IK, Graham R, Garcia CK, Hobbs HH
Nature genetics 2005 Feb;37(2):161-5
Nature genetics 2005 Feb;37(2):161-5
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - Analysis of PCSK9 in McA cell lysates: untransfected (1), transfected with wild type human PCSK9 (2), transfected with S127R human PCSK9 (3) using NB300-959 (0.2ug/ml). Data kindly provided by Dr. X-M Sun, Hammersmith Hospital, London.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
