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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [28]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AF591 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Mer Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Mer in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human Mer is observed and less than 1% cross-reactivity with recombinant mouse (rm) Axl and rmDtk is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q60805- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Pan-TAM Tyrosine Kinase Inhibitor BMS-777607 Enhances Anti-PD-1 mAb Efficacy in a Murine Model of Triple-Negative Breast Cancer.
Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage.
Sitravatinib potentiates immune checkpoint blockade in refractory cancer models.
LXR Suppresses Inflammatory Gene Expression and Neutrophil Migration through cis-Repression and Cholesterol Efflux.
Macrophage deficiency of miR-21 promotes apoptosis, plaque necrosis, and vascular inflammation during atherogenesis.
Schwann cells use TAM receptor-mediated phagocytosis in addition to autophagy to clear myelin in a mouse model of nerve injury.
Migrating Interneurons Secrete Fractalkine to Promote Oligodendrocyte Formation in the Developing Mammalian Brain.
Sleep Loss Promotes Astrocytic Phagocytosis and Microglial Activation in Mouse Cerebral Cortex.
GAS6 is a key homeostatic immunological regulator of host-commensal interactions in the oral mucosa.
MicroRNA-126 overexpression rescues diabetes-induced impairment in efferocytosis of apoptotic cardiomyocytes.
MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation.
Liver X receptor and STAT1 cooperate downstream of Gas6/Mer to induce anti-inflammatory arginase 2 expression in macrophages.
TAM receptors regulate multiple features of microglial physiology.
Collecting lymphatic vessel permeability facilitates adipose tissue inflammation and distribution of antigen to lymph node-homing adipose tissue dendritic cells.
Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration.
Diversification of TAM receptor tyrosine kinase function.
A new mouse model for female genital schistosomiasis.
Ly6Chi monocyte recruitment is responsible for Th2 associated host-protective macrophage accumulation in liver inflammation due to schistosomiasis.
Minimal differentiation of classical monocytes as they survey steady-state tissues and transport antigen to lymph nodes.
Cross-phosphorylation, signaling and proliferative functions of the Tyro3 and Axl receptors in Rat2 cells.
Inhibiting Mer receptor tyrosine kinase suppresses STAT1, SOCS1/3, and NF-κB activation and enhances inflammatory responses in lipopolysaccharide-induced acute lung injury.
Complement component C1q regulates macrophage expression of Mer tyrosine kinase to promote clearance of apoptotic cells.
Protein S protects neurons from excitotoxic injury by activating the TAM receptor Tyro3-phosphatidylinositol 3-kinase-Akt pathway through its sex hormone-binding globulin-like region.
Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor.
Mertk receptor mutation reduces efferocytosis efficiency and promotes apoptotic cell accumulation and plaque necrosis in atherosclerotic lesions of apoe-/- mice.
Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.
A soluble form of the Mer receptor tyrosine kinase inhibits macrophage clearance of apoptotic cells and platelet aggregation.
Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.
Kasikara C, Davra V, Calianese D, Geng K, Spires TE, Quigley M, Wichroski M, Sriram G, Suarez-Lopez L, Yaffe MB, Kotenko SV, De Lorenzo MS, Birge RB
Cancer research 2019 May 15;79(10):2669-2683
Cancer research 2019 May 15;79(10):2669-2683
Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage.
Aran D, Looney AP, Liu L, Wu E, Fong V, Hsu A, Chak S, Naikawadi RP, Wolters PJ, Abate AR, Butte AJ, Bhattacharya M
Nature immunology 2019 Feb;20(2):163-172
Nature immunology 2019 Feb;20(2):163-172
Sitravatinib potentiates immune checkpoint blockade in refractory cancer models.
Du W, Huang H, Sorrelle N, Brekken RA
JCI insight 2018 Nov 2;3(21)
JCI insight 2018 Nov 2;3(21)
LXR Suppresses Inflammatory Gene Expression and Neutrophil Migration through cis-Repression and Cholesterol Efflux.
Thomas DG, Doran AC, Fotakis P, Westerterp M, Antonson P, Jiang H, Jiang XC, Gustafsson JÅ, Tabas I, Tall AR
Cell reports 2018 Dec 26;25(13):3774-3785.e4
Cell reports 2018 Dec 26;25(13):3774-3785.e4
Macrophage deficiency of miR-21 promotes apoptosis, plaque necrosis, and vascular inflammation during atherogenesis.
Canfrán-Duque A, Rotllan N, Zhang X, Fernández-Fuertes M, Ramírez-Hidalgo C, Araldi E, Daimiel L, Busto R, Fernández-Hernando C, Suárez Y
EMBO molecular medicine 2017 Sep;9(9):1244-1262
EMBO molecular medicine 2017 Sep;9(9):1244-1262
Schwann cells use TAM receptor-mediated phagocytosis in addition to autophagy to clear myelin in a mouse model of nerve injury.
Brosius Lutz A, Chung WS, Sloan SA, Carson GA, Zhou L, Lovelett E, Posada S, Zuchero JB, Barres BA
Proceedings of the National Academy of Sciences of the United States of America 2017 Sep 19;114(38):E8072-E8080
Proceedings of the National Academy of Sciences of the United States of America 2017 Sep 19;114(38):E8072-E8080
Migrating Interneurons Secrete Fractalkine to Promote Oligodendrocyte Formation in the Developing Mammalian Brain.
Voronova A, Yuzwa SA, Wang BS, Zahr S, Syal C, Wang J, Kaplan DR, Miller FD
Neuron 2017 May 3;94(3):500-516.e9
Neuron 2017 May 3;94(3):500-516.e9
Sleep Loss Promotes Astrocytic Phagocytosis and Microglial Activation in Mouse Cerebral Cortex.
Bellesi M, de Vivo L, Chini M, Gilli F, Tononi G, Cirelli C
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 May 24;37(21):5263-5273
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 May 24;37(21):5263-5273
GAS6 is a key homeostatic immunological regulator of host-commensal interactions in the oral mucosa.
Nassar M, Tabib Y, Capucha T, Mizraji G, Nir T, Pevsner-Fischer M, Zilberman-Schapira G, Heyman O, Nussbaum G, Bercovier H, Wilensky A, Elinav E, Burstyn-Cohen T, Hovav AH
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 17;114(3):E337-E346
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 17;114(3):E337-E346
MicroRNA-126 overexpression rescues diabetes-induced impairment in efferocytosis of apoptotic cardiomyocytes.
Suresh Babu S, Thandavarayan RA, Joladarashi D, Jeyabal P, Krishnamurthy S, Bhimaraj A, Youker KA, Krishnamurthy P
Scientific reports 2016 Nov 9;6:36207
Scientific reports 2016 Nov 9;6:36207
MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation.
Cai B, Thorp EB, Doran AC, Subramanian M, Sansbury BE, Lin CS, Spite M, Fredman G, Tabas I
Proceedings of the National Academy of Sciences of the United States of America 2016 Jun 7;113(23):6526-31
Proceedings of the National Academy of Sciences of the United States of America 2016 Jun 7;113(23):6526-31
Liver X receptor and STAT1 cooperate downstream of Gas6/Mer to induce anti-inflammatory arginase 2 expression in macrophages.
Kim SY, Lim EJ, Yoon YS, Ahn YH, Park EM, Kim HS, Kang JL
Scientific reports 2016 Jul 13;6:29673
Scientific reports 2016 Jul 13;6:29673
TAM receptors regulate multiple features of microglial physiology.
Fourgeaud L, Través PG, Tufail Y, Leal-Bailey H, Lew ED, Burrola PG, Callaway P, Zagórska A, Rothlin CV, Nimmerjahn A, Lemke G
Nature 2016 Apr 14;532(7598):240-244
Nature 2016 Apr 14;532(7598):240-244
Collecting lymphatic vessel permeability facilitates adipose tissue inflammation and distribution of antigen to lymph node-homing adipose tissue dendritic cells.
Kuan EL, Ivanov S, Bridenbaugh EA, Victora G, Wang W, Childs EW, Platt AM, Jakubzick CV, Mason RJ, Gashev AA, Nussenzweig M, Swartz MA, Dustin ML, Zawieja DC, Randolph GJ
Journal of immunology (Baltimore, Md. : 1950) 2015 Jun 1;194(11):5200-10
Journal of immunology (Baltimore, Md. : 1950) 2015 Jun 1;194(11):5200-10
Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration.
Levy O, Calippe B, Lavalette S, Hu SJ, Raoul W, Dominguez E, Housset M, Paques M, Sahel JA, Bemelmans AP, Combadiere C, Guillonneau X, Sennlaub F
EMBO molecular medicine 2015 Feb;7(2):211-26
EMBO molecular medicine 2015 Feb;7(2):211-26
Diversification of TAM receptor tyrosine kinase function.
Zagórska A, Través PG, Lew ED, Dransfield I, Lemke G
Nature immunology 2014 Oct;15(10):920-8
Nature immunology 2014 Oct;15(10):920-8
A new mouse model for female genital schistosomiasis.
Richardson ML, Fu CL, Pennington LF, Honeycutt JD, Odegaard JI, Hsieh YJ, Hammam O, Conti SL, Hsieh MH
PLoS neglected tropical diseases 2014 May;8(5):e2825
PLoS neglected tropical diseases 2014 May;8(5):e2825
Ly6Chi monocyte recruitment is responsible for Th2 associated host-protective macrophage accumulation in liver inflammation due to schistosomiasis.
Nascimento M, Huang SC, Smith A, Everts B, Lam W, Bassity E, Gautier EL, Randolph GJ, Pearce EJ
PLoS pathogens 2014 Aug;10(8):e1004282
PLoS pathogens 2014 Aug;10(8):e1004282
Minimal differentiation of classical monocytes as they survey steady-state tissues and transport antigen to lymph nodes.
Jakubzick C, Gautier EL, Gibbings SL, Sojka DK, Schlitzer A, Johnson TE, Ivanov S, Duan Q, Bala S, Condon T, van Rooijen N, Grainger JR, Belkaid Y, Ma'ayan A, Riches DW, Yokoyama WM, Ginhoux F, Henson PM, Randolph GJ
Immunity 2013 Sep 19;39(3):599-610
Immunity 2013 Sep 19;39(3):599-610
Cross-phosphorylation, signaling and proliferative functions of the Tyro3 and Axl receptors in Rat2 cells.
Brown JE, Krodel M, Pazos M, Lai C, Prieto AL
PloS one 2012;7(5):e36800
PloS one 2012;7(5):e36800
Inhibiting Mer receptor tyrosine kinase suppresses STAT1, SOCS1/3, and NF-κB activation and enhances inflammatory responses in lipopolysaccharide-induced acute lung injury.
Lee YJ, Han JY, Byun J, Park HJ, Park EM, Chong YH, Cho MS, Kang JL
Journal of leukocyte biology 2012 Jun;91(6):921-32
Journal of leukocyte biology 2012 Jun;91(6):921-32
Complement component C1q regulates macrophage expression of Mer tyrosine kinase to promote clearance of apoptotic cells.
Galvan MD, Foreman DB, Zeng E, Tan JC, Bohlson SS
Journal of immunology (Baltimore, Md. : 1950) 2012 Apr 15;188(8):3716-23
Journal of immunology (Baltimore, Md. : 1950) 2012 Apr 15;188(8):3716-23
Protein S protects neurons from excitotoxic injury by activating the TAM receptor Tyro3-phosphatidylinositol 3-kinase-Akt pathway through its sex hormone-binding globulin-like region.
Zhong Z, Wang Y, Guo H, Sagare A, Fernández JA, Bell RD, Barrett TM, Griffin JH, Freeman RS, Zlokovic BV
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Nov 17;30(46):15521-34
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Nov 17;30(46):15521-34
Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor.
Zhu D, Wang Y, Singh I, Bell RD, Deane R, Zhong Z, Sagare A, Winkler EA, Zlokovic BV
Blood 2010 Jun 10;115(23):4963-72
Blood 2010 Jun 10;115(23):4963-72
Mertk receptor mutation reduces efferocytosis efficiency and promotes apoptotic cell accumulation and plaque necrosis in atherosclerotic lesions of apoe-/- mice.
Thorp E, Cui D, Schrijvers DM, Kuriakose G, Tabas I
Arteriosclerosis, thrombosis, and vascular biology 2008 Aug;28(8):1421-8
Arteriosclerosis, thrombosis, and vascular biology 2008 Aug;28(8):1421-8
Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.
Chang Y, Finnemann SC
Journal of cell science 2007 Sep 1;120(Pt 17):3053-63
Journal of cell science 2007 Sep 1;120(Pt 17):3053-63
A soluble form of the Mer receptor tyrosine kinase inhibits macrophage clearance of apoptotic cells and platelet aggregation.
Sather S, Kenyon KD, Lefkowitz JB, Liang X, Varnum BC, Henson PM, Graham DK
Blood 2007 Feb 1;109(3):1026-33
Blood 2007 Feb 1;109(3):1026-33
Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.
Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA
Journal of thrombosis and haemostasis : JTH 2005 Apr;3(4):733-41
Journal of thrombosis and haemostasis : JTH 2005 Apr;3(4):733-41
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Mer in Mouse Testis. Mer was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sperm cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mer in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
