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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [5]
- Other assay [3]
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- Product number
- PA5-57331 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: HKRQHPGRRE DEASWSVDVT QHKRQHPGRR SPWLAYAVPK RQHPGRRLAD PKAQRSWEE
- Concentration
- 0.48 mg/mL
Submitted references Differences between rats and mice in the leptin action on the paraventricular nucleus of the hypothalamus: Implications for the regulation of the hypothalamic-pituitary-thyroid axis.
Campos AMP, Teixeira PDS, Wasinski F, Klein MO, Bittencourt JC, Metzger M, Donato J Jr
Journal of neuroendocrinology 2020 Sep;32(9):e12895
Journal of neuroendocrinology 2020 Sep;32(9):e12895
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of TRH in human hypothalamus using a TRH Polyclonal Antibody (Product # PA5-57331) shows strong positivity neuronal processes and subsets of neuronal cells and .
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of TRH in human cervix, uterine using TRH Polyclonal Antibody (Product # PA5-57331) shows moderate to strong cytoplasmic positivity in glandular cells.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of TRH in human endometrium using TRH Polyclonal Antibody (Product # PA5-57331) shows weak to moderate cytoplasmic positivity in glandular cells.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of TRH in human testis using TRH Polyclonal Antibody (Product # PA5-57331) shows no positivity in cells in seminiferous ducts as expected.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of TRH in human skeletal muscle using TRH Polyclonal Antibody (Product # PA5-57331) shows no positivity in myocytes as expected.
Supportive validation
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- Experimental details
- 2 Figure Thyrotrophin-releasing hormone (TRH) neurones in the paraventricular nucleus of the hypothalamus of rats are directly responsive to leptin. A-F, Epifluorescence photomicrographs showing the co-localisation between phosphorylated signal transducer and activator of transcription 3 (pSTAT3) (green staining) and proTRH (red staining) in rats that received an i.c.v. injection of leptin (A-C) and in rats that did not receive leptin injection (D-F). The arrows indicate examples of double-labelled neurones. Abbreviations: 3V, third ventricle; ARH, arcuate nucleus; PVH, paraventricular nucleus of the hypothalamus; VMH, ventromedial nucleus. Scale bar = 50 um in (A) and (D); 20 um in (B) and (E); 100 um in (C) and (F)
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- Invitrogen Antibodies (provider)
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- 3 Figure By contrast to rats, thyrotrophin-releasing hormone (TRH) neurones in the hypothalamic paraventricular nucleus of mice are mostly unresponsive to leptin. A-I, Epifluorescence photomicrographs showing the co-localisation between phosphorylated signal transducer and activator of transcription 3 (pSTAT3) (green staining) and proTRH (red staining) in mice that received an i.p. injection of leptin (A-C) and in mice that received an i.c.v. injection of leptin (D-F), as well as the co-localisation between leptin receptor (LepR) and proTRH in LepR-reporter mice (G-I). The arrows indicate examples of double-labelled neurones, whereas arrowheads indicate leptin-responsive neurones that do not express proTRH. Abbreviations: 3V, third ventricle; ARH, arcuate nucleus; PVH, paraventricular nucleus of the hypothalamus; VMH, ventromedial nucleus. Scale bar = 50 um in (A), (D) and (G); 20 um in (B), (E) and (H); 100 um in (C), (F) and (I)
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 4 Figure Leptin induces phosphorylation of the cAMP response element binding protein (pCREB) in thyrotrophin-releasing hormone (TRH) neurones of rats, but not in mice. A-C, Co-localisation between pCREB (green staining) and proTRH (red staining) in the paraventricular nucleus of the hypothalamus (PVH) of rats (n = 3 per group). D-F, Co-localisation between pCREB and proTRH in the PVH of mice (n = 4-6 per group). The arrows indicate examples of double-labelled neurones. Abbreviation: 3V, third ventricle. Scale bar = 100 um. *** P = 0.0007
