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- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF1298 - Provider product page
- Provider
- R&D Systems
- Product name
- Human ULBP-2/5/6 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human ULBP-2, ULBP-5, and RAET1L/ULBP-6 in Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) ULBP-1, rhULBP-3, and rhULBP-4 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9BZM5- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Natural Killer Cell Evasion Is Essential for Infection by Rhesus Cytomegalovirus.
PRL-3 mediates the protein maturation of ULBP2 by regulating the tyrosine phosphorylation of HSP60.
MICA/B and ULBP1 NKG2D ligands are independent predictors of good prognosis in cervical cancer.
Tumor suppressive microRNAs miR-34a/c control cancer cell expression of ULBP2, a stress-induced ligand of the natural killer cell receptor NKG2D.
Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection.
The human NKG2D ligand ULBP2 can be expressed at the cell surface with or without a GPI anchor and both forms can activate NK cells.
Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform.
Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells.
Sturgill ER, Malouli D, Hansen SG, Burwitz BJ, Seo S, Schneider CL, Womack JL, Verweij MC, Ventura AB, Bhusari A, Jeffries KM, Legasse AW, Axthelm MK, Hudson AW, Sacha JB, Picker LJ, Früh K
PLoS pathogens 2016 Aug;12(8):e1005868
PLoS pathogens 2016 Aug;12(8):e1005868
PRL-3 mediates the protein maturation of ULBP2 by regulating the tyrosine phosphorylation of HSP60.
Leung WH, Vong QP, Lin W, Bouck D, Wendt S, Sullivan E, Li Y, Bari R, Chen T, Leung W
Journal of immunology (Baltimore, Md. : 1950) 2015 Mar 15;194(6):2930-41
Journal of immunology (Baltimore, Md. : 1950) 2015 Mar 15;194(6):2930-41
MICA/B and ULBP1 NKG2D ligands are independent predictors of good prognosis in cervical cancer.
Cho H, Chung JY, Kim S, Braunschweig T, Kang TH, Kim J, Chung EJ, Hewitt SM, Kim JH
BMC cancer 2014 Dec 15;14:957
BMC cancer 2014 Dec 15;14:957
Tumor suppressive microRNAs miR-34a/c control cancer cell expression of ULBP2, a stress-induced ligand of the natural killer cell receptor NKG2D.
Heinemann A, Zhao F, Pechlivanis S, Eberle J, Steinle A, Diederichs S, Schadendorf D, Paschen A
Cancer research 2012 Jan 15;72(2):460-71
Cancer research 2012 Jan 15;72(2):460-71
Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection.
Chang YT, Wu CC, Shyr YM, Chen TC, Hwang TL, Yeh TS, Chang KP, Liu HP, Liu YL, Tsai MH, Chang YS, Yu JS
PloS one 2011;6(5):e20029
PloS one 2011;6(5):e20029
The human NKG2D ligand ULBP2 can be expressed at the cell surface with or without a GPI anchor and both forms can activate NK cells.
Fernández-Messina L, Ashiru O, Agüera-González S, Reyburn HT, Valés-Gómez M
Journal of cell science 2011 Feb 1;124(Pt 3):321-7
Journal of cell science 2011 Feb 1;124(Pt 3):321-7
Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform.
Ohashi M, Eagle RA, Trowsdale J
The Journal of biological chemistry 2010 May 28;285(22):16408-15
The Journal of biological chemistry 2010 May 28;285(22):16408-15
Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells.
Schwinn N, Vokhminova D, Sucker A, Textor S, Striegel S, Moll I, Nausch N, Tuettenberg J, Steinle A, Cerwenka A, Schadendorf D, Paschen A
International journal of cancer 2009 Apr 1;124(7):1594-604
International journal of cancer 2009 Apr 1;124(7):1594-604
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- ULBP-2/5/6 in Human Colon Cancer Tissue. ULBP-2/5/6 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using 10 µg/mL Goat Anti-Human ULBP-2/5/6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1298) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
